【作者】 郑洋； 王金刚； 龚束芳； 樊金萍； 车代弟；

【Author】 ZHENG Yang WANG Jin-gang GONG Shu-fang FAN Jin-ping CHE Dai-di (College of Horticultve,North-east Agricultire Univeristy,Harbin 150030)

【机构】 东北农业大学园艺学院；

【摘要】 以唐菖蒲球茎芽切片为外植体,经体细胞胚发生途径,进行胚性愈伤组织诱导、胚状体的诱导、胚状体发育过程及植株再生的研究。经研究认为胚性愈伤组织诱导培养基为 MS+2,4-D4.0mg/L+6-BA0.5mg/L, 诱导率为95%;胚状体诱导培养基为 MS+2,4-D1.0mg/L+TDZ0.2mg/L,诱导率为68.3%;将产生的胚状体首先接种于 MS 培养基使其充分发育,之后转入 MS+6-BA2.0mg/L 培养基中诱导发芽,在转入 MS 培养基中使其形成完整植株。采用石蜡切片法和临时压片法对胚状体的发育过程进行了观察发现,首先外植体表层薄壁细胞经脱分化恢复分生能力,形成愈伤组织,随后在愈伤组织表面形成许多瘤状突起即胚性细胞团,胚性细胞团继续发育成球形胚、盾形胚,最后发育成熟形成完整植株。更多还原

【Abstract】 Taking the slice of ghulious(corms as explants,the regenerated way of plant was selected somatic embryogene- sis.result showed that:MS basal medium supplemented with 2,4-D4mg/L and 6BA0.5mg/L was advantageous to callus induc- tion,The frequency of callus induction was 95(;MS+2,4-D1.0mg/L+TDZ0.2 mg/L was the best culture medium that the induction of somatic embryo,the rate was 57%.After that there was three steps to complete the culture:First,the regenera- ted somatic embryo were cultured in the MS medium to growth enough.;Second,the growful somatic embryo was cultured in the MS+6-BA2.0mg/L to induce buds;Third,the regenerated plants were obtained in the MS medium.The appearance and growth of somatic embryo were observed through paraffin slices and press slices.Histological observations showed that paren- chyma cells first experienced dedifferentiation and followed with callus-forming,ensued with embryngenic cell masses which like a tubercular structure,and these embryogenic cell masses experienced globular,scutellate stages and developed into whole plantlets.更多还原