【作者】 王丽萍； 郑一凡； 杨军； 章军； 张群卫； 朱心强；

【Author】 WANG Liping1, ZHENG Yifan 1, YANG Jun 1, ZHANG Jun 1, ZHANG Qunwei 2, ZHU XinQiang 1* (1. Department of Toxicology, Zhejiang University School of Public Health, Hangzhou 310058, Zhejiang, China; 2 . Department of Environmental and Occupational Health Sciences, School of Public Health and Information Sciences, University of Louisville, Louisville, Kentucky 40202, USA)

【机构】 浙江大学公共卫生学院毒理实验室； 美国路易斯维尔大学公众卫生与情报科学学院环境与职业健康学系；

【摘要】 量子点(QDs)在纳米医学领域具有广泛的应用前景,但是其对健康的影响并不明确。本研究中,我们主要观察了碲化镉量子点(CdTeQDs)对人脐静脉内皮细胞(HUVECs)的细胞毒性和遗传毒性,探讨QDs潜在的心血管毒性危害。结果发现QDs对HUVECs具有剂量依赖的细胞生长抑制效应。用γH2AX焦点形成检测DNA损伤,发现1、10和50μg/mlQDs作用于HUVECs12h可以显著诱导γH2AX的焦点形成并呈剂量效应关系,流式细胞仪分析结果也显示了同样的结果。进一步研究发现,QDs处理可以诱导活性氧(ROS)的产生,而自由基清除剂N-乙酰半胱氨酸(NAC)可以显著抑制QDs诱导的ROS的产生并减少γH2AX的焦点形成。因此,在本研究条件下,CdTeQDs对HUVECs具有细胞毒性和遗传毒性效应,诱导ROS产生可能是QDsDNA损伤作用的机制之一。更多还原

【Abstract】 Quantum dots (QDs) holds great potential in nanomedicine, however, the health effects of QDs are largely unknown. In the present study, the cytotoxicity and genotoxicity of CdTe QDs were examined in human umbilical vein endothelial cells (HUVECs) to identify the possible cardiovascular risks of QDs. It was found that QDs exhibited a dose-dependent inhibitory effect on cell growth. Using γH2AX foci formation as an indicator for DNA damage, it was shown that QDs at 1, 10, and 50 μg/ml induced formation of γH2AX foci in HUVECs at 12 h post-treatment in a dose-dependent manner. Flow cytometry analysis showed similar results. QDs treatment also induced the generation of reactive oxygen species (ROS). Pre-treatment with N-acetyl-cystein (NAC), an ROS scavenger, can inhibit the induction of ROS by QDs, as well as the formation of γH2AX foci. Taken together, the data indicate that CdTe QDs have cytotoxic and genotoxic effects on HUVECs, and that ROS generation might be involved in QDs induced DNA damage.更多还原