【作者】 杨霞； 闫爱华； 丁兰平； 宫亚欧； 杨胜利；

【Author】 YANG Xia YAN Aihua DING Lanping GONG Yaou YANG Shengli (1.Department of Esophageal Cancer, College of Basic Medicine, Zhengzhou University, Zhengzhou Henan 450052 )

【机构】 郑州大学基础医学院食管癌研究室；

【摘要】 背景与目的:探讨食管鳞状细胞癌中O6-甲基鸟嘌呤-DNA甲基转移酶(O6-methylguanine DNA methyltransferase,MGMT)基因和p16基因启动子的甲基化情况,并对二者甲基化的频率进行统计学分析,从而了解这两个基因启动子甲基化与食管癌发生的关系。材料与方法:采用甲基化特异性PCR(MSP)方法分别对44例食管鳞状细胞癌组织进行MGMT基因和p16基因的甲基化检测并对其甲基化频率分别进行差别性检验和关联性分析。结果:在44例食管鳞癌组织中,有18例(40.9%)出现MGMT基因启动子甲基化,23例(52.3%)出现p16基因启动子甲基化,7例(14.9%)两基因均出现甲基化,10例(22.7%)两基因均未出现甲基化。MGMT和p16基因启动子甲基化率差异无统计学意义(P>0.05)MGMT和p16基因启动子甲基化无明显相关关系(P>0.05)。结论:MGMT和p16基因启动子甲基化均可能与食管鳞状细胞癌发生、发展过程密切相关,但二者是相互独立进行的。更多还原

【Abstract】 Background and Aim: To investigate the promoter methylation state of MGMT (O6-methylguanine DNA methyltransferase) gene and p16 gene in esophageal squamous cell carcinoma, and analyze the methylation frequency of the two genes, thereby find out the relationship between the methylation of the promoters of MGMT gene and p16 gene in esophageal squamous cell carcinoma. Materials and Methods: The methylation of the promoter of MGMT gene and p16 gene was detected respectively by methylation-specific PCR in 44 esophageal carcinoma tissues. and carry out respectively distictiveness test and associativity analysis to methylation frequency of two genes using χ2 test. Results: Among the 44 esophageal squamous cell carcinomas, MGMT gene methylation was detected in 18 cases; p16 gene methylation was detected in 23 cases;both MGMT and p16 gene methylation were detected in 7 cases; Neither MGMT nor p16 gene methylation was detected in 10 cases. The difference of the methylation frequency of the two genes is nonsignificant(P>0.05)and carry out respectively distictiveness test and associativity analysis to methylation frequency of two genes using χ2 test. Conclusions :The methylations of the promoter of MGMT gene and p16 gene may be both closely associated with the initiation and progression of the esophageal squamous cell carcinoma, but the methylations of the promoter of the two genes take place independently each other, and there are no directly relation in them.更多还原