【作者】 苌收伟； 秦广雍； 余增亮； 吴跃进； 程玉红； 王娟； 苏明杰；

【Author】 Chang Shouwei1,Qin Guangyong2,Yu Zengliang3 Wu Yuejin3,Cheng Yuhong1,Wang Juan1,Su Mingjie2 (1.Henan Province Hebi Agronomy Research Institute, Hebi 458030, Henan; 2.Henan Province Key Laboratory of Ion Beam Bioengineering, Zhengzhou University, Zhengzhou 450052, Henan; 3.Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences, Hefei 230031, Anhui)

【机构】 河南省鹤壁市农科所； 郑州大学离子束生物工程省重点实验室； 中国科学院离子束生物工程学重点实验室；

【摘要】 采用离子束介导法将大豆DNA导入中育5号、淮阴9628两个小麦品种。结果表明:T1代获得高蛋白(>15%)单株114株,最高株蛋白质含量为21.44%,明显提高了小麦单株蛋白质含量,而且,这些高蛋白性状变异,从T1到T3代大都可以遗传,部分系统在T3代基本稳定,说明离子束介导外源DNA,明显提高了基因转移和诱变作用,变异性状可稳定遗传,有可能成为实现超级小麦育种突破的一条最有效途径。更多还原

【Abstract】 The method of low energy ion beam-mediated transformation was adopted to transfer the soybean DNA into two wheat varieties ‘zhongyu 5’and ‘huaiyin 9628’.The results suggested that 114 high-protein individuals(protein content >15%) were obtained from T1,and the highest protein content reached 21.44%. The protein content of wheat individuals was greatly increased. The high-protein character could be mostly inherited from T1 to T3, and the character of some systems in T3 was stable basically. It was showed that the method of low energy ion beam-mediated transformation obviously enhanced the transgene and mutagenic effect, and the variant character could be inherited stably. So this method may become to a most efficient way to realize the breakthrough of wheat breeding.更多还原