【作者】 徐红； 范少光；

【Author】 Xu HONG AND FAN SHAO-GUANG (Department of Physiology, Beijing Medical University)

【机构】 北京医科大学生理教研室；

【摘要】 本室以前的工作表明:电针(2Hz,3V,30min/d)刺激SD大鼠双侧足三里-三阴交,5d 后,大鼠血清中产生出淋巴细胞转化抑制因子,本工作对此抑制因子的作用机制进行了初步研究,主要结果如下:(1)电针大鼠的血清不仅显著抑制Con A刺激的小鼠淋巴结T淋巴细胞转化,还可显著抵制Con A刺激的小鼠胸腺细胞和脾脏T淋巴细胞转化;同时也发现电针大鼠的血清能显著抑制脂多糖(LPS)刺激的小鼠淋巴结B淋巴细胞转化。提示此淋巴细胞转化抑制因子对不同淋巴器官及不同类型的淋巴细胞无选择性作用。(2)将电针大鼠的血清同小鼠淋巴结细胞培养1h,电针大鼠的血清就可显著抑制Con A刺激的T淋巴细胞转化;将小鼠淋巴结细胞同Con A预培养30min,电针大鼠的血清的抑制作用便消失,提示电针大鼠血清中淋巴细胞转化抑制因子作用于Con A刺激T淋巴细胞活化的早期阶段,同时也排除了此抑制因子的细胞毒作用。(3)电针大鼠的血清显著抑制蛋白激酶C(PKC)激活剂PMA 和PMA加Ca2+通道A23187刺激的小鼠淋巴结细胞转化,提示淋巴细胞转化抑制因子通过抑制PKC的活性或抑制PKC介导的细胞活化通路,抑制有丝分裂原刺激的淋巴细胞转化。更多还原

【Abstract】 Previous reports showed that EA stimulation (3V, 2Hz, 30 min / d, 5 d) induced the production of one or more lymphocyte proliferation-inhibitory factor (s) in the rat serum. In this paper, the mechanisms of the action for the inhibitory factor (s) to suppress lymphocyte proliferation were studied. (1) the lymphocytes from different immune organs of the mice were prepared and cultured with the rat serum stimulated by EA. The results show that the serum not only inhibited the mouse lymphonodus T cell proliferation induced by Con A, but also inhibited the mouse thymocyte and spleen T cell proliferation induced by Con A. When, B cells were stimulated by LPS, the proliferative effect can also be inhibited significantly by the rat serum stimulated by EA. This implies that the effect of the lymphocyte proliferation-inhibitory factor(s) has no specificity. (2) Incubation of the mouse lymphonodus cell with serum for one hour is enough to cause an inhibitory effect on Con A stimulated lymphocyte proliferation. However, no inhibitory effect was observed if the mouse lymphonodus cells were incubated with Con A for 15min or 30 min befere the addition of rat serum. The results demonstrate that the lymphocyte proliferation-inhibitory factor(s) act on the early events of T lymphocyte activation induced by Con A. (3) Protein kinase C (PKC) is a key link in the activation of T and B lymphocyte proliferation by Con A and LPS respectively. So it would be interesting to learn whether the inhibitory effect of the lymphocyte proliferation-inhibitory factior(s) is caused by the inhibition of PKC activity. It was-observed that the serum indeed inhibits the lymphocyte proliferation induced by PMA (a PKC activator) or in combination with Ca2+ ionophore A23187. These results suggest that the inhibitory effect of the lymphocyte proliferation-inhibitory factor(s) might be mediated by some direct inhibition of PKC activity or indirectly through PKC activated pathways.更多还原