【作者】 丁壮； Shinji TAKAI； Hiroo MADARAME； 常爽； 黄海楠； 霍晓伟； 高明华； 谭忠田； 高双成； Fumiko HATORI； Yukako SASAKI； Tsutomu KAKUDA； Shiro TSUBAKI；

【Author】 Ding Zhuang, Shinji TAKAI, Hiroo MADARAME, Chang Shuang, Huang Hai-nan, Huo Xiao-wei , Gao Ming-hua, Tan Zhong-tian, Gao Shuang-cheng ,Fumiko HATORI, Yukako SASAKI, Tsutomu KAKUDA,Shiro TSUBAKI (College of Animal and Veterinary Science, Jilin University Department of Preventive Veterinary Medicine, Changchun, Jilin 130062, China; Department of Animal Hygienge, School of Veterinary Medicine and Animal Science, Kitasato University, Tawada, Aomori 034-8628, Japan; Veterinary Teaching Hospital, Azab, University, 1-17-71 Fuchinobe, Sagamihara-shi, Kanagawa-ken, 229-8501, Japan; College of Animal Science and Technology, Inner Mongolia University for the Nationalities, Tongliao, Inner Mongolia 028043, China; Zalantun School of Animal and Veterinary Science, Inner Mongolia 162650, China; ManZhouLi City Veterinary Station, Inner Mongolia 021400, China; ChenQi Veterinary Station, Inner Mongolia 021500, China)

【机构】 吉林大学畜牧兽医学院； 日本北里大学兽医畜产学部兽医卫生学教研室； 麻布大学兽医学部附属动物病院； 中国内蒙古民族大学动物科学技术学院； 内蒙古扎兰屯农牧学校； 满洲里市兽医站； 陈旗兽医站；

【摘要】 中国内蒙古自治区饲育马土壤环境中马红球菌分离与分布知之甚少。在中国内蒙古通辽近郊、内蒙古南部锡林郭勒盟大草原、内蒙古东部呼伦贝尔大草原等马场中收集了108份土壤样品。从锡林郭勒盟大草原和呼伦贝尔大草原马红球菌的分离率为25．9％-30．0％,通辽近郊土壤分离率高达82．3 ％。这说明在通辽近郊马红球菌的分离菌数是草原土壤中马红球菌分离菌数的10倍。应用PCR技术来检测488株毒力相关基因,分子量分别为15-17kDa(VapA)和20kDa(VapB)。对于毒力相关基因来说所有的分离株都是阴性的。这些无毒力分离株的质粒资料显示,各种大小的潜在质粒呈现发生率为13．3％-21．5％。与近年来的一些研究相比,目前的研究结果显示马红球菌在蒙古乌兰巴托,内蒙古马群中不存在。这表明两个研究结果的差异在于蒙古的游牧和内蒙古非游牧生活。更多还原

【Abstract】 Little is known about the distribution of Rhodococcus equi in the soil environment of native horses in China. One hundred and eight soil samples were collected from native-horse farms in the Hulun Beier grasslands of eastern Mongolia, the Xilin Goler grasslands of southern Mongolia, and Tongliao City in Inner Mongolia, China. The isolation rates of R. equi from soil samples from the Hulun Beier and Xilin Goler grasslands ranged from 25.9% to 30.0%. In contrast, isolation rates from soil samples from Tongliao City was as high as 82.3% and the mean number of R. equi in soil samples from Tongliao City was 10 times more than those of samples from the grasslands. The 488 isolates were examined using PCR for the presence of genes that encode virulence-associated 15-17kDa antigen protein (VapA) and the 20kDa antigen protein (VapB). All isolates were negative for virulence-associated proteins. Plasmid profiles of these avirulent isolates showed that cryptic plasmids of various sizes were present with an incidence of 13.3% to 21.5%. The results of the present study contrast with those of our recent study (J. Vet. Med. Sci. 67:611-613, 2005), in which we reported that our recent that R. equi was absent from Mongolian horses in Ulaanbaatar, Mongolia. It is suggested that the difference between the results of these two studies is due to the mobile pasturing system in Mongolia and nonmobile pasturing system in Inner Mongolia.更多还原