【作者】 乔永； 黄治国； 李齐发； 刘振山； 代蓉； 谢庄； 郝称莉； 刘红林；

【Author】 QIAO Yong, HUANG Zhi-guo, LI Qi-fa, LIU Zhen-shan, DAI Rong, XIE Zhuang, LIU Hong-lin, (College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu ,210095 , China; Sichuan university of science & engineering, Zigong Sichuan, 643000, China; Xinjiang Reclamation Science Institute,Shihezi 832001, China;)

【机构】 南京农业大学动物科技学院； 新疆农垦科学院畜牧兽医研究所；

【摘要】 本研究选取2、30、60、90和120日龄的雄性哈萨克羊和新疆细毛羊各6只(120日龄只有新疆细毛羊),屠宰后取背最长肌,用索氏抽提法检测肌内脂肪(Intamuscular fat,IMF)含量,用荧光实时定量PCR法检测肌肉脂蛋白脂酶(lipoprotein lipase,LPL)基因mRNA表达水平, 分析在不同时期的变化及其与肌内脂肪含量的关系。结果表明:(1)随着日龄的增加,雄性哈萨克羊的IMF含量持续上升,各生长时期差异显著(P<0．05),而新疆细毛羊的IMF含量在各生长时期无显著差异(P>0．05)。雄性哈萨克羊的IMF含量30-90日龄期间极显著高于新疆细毛羊 (P<0．01)。(2)雄性哈萨克羊肌肉LPL基因的表达量在2日龄时最高,60日龄时降到最低,然后逐渐上升,2日龄时LPL表达量极显著高于其它日龄(P<0．01)。新疆细毛羊的表达量在2日龄时较低,30日龄时升到最高,随后下降,到90日龄时降到最低,然后又逐渐上升;30日龄表达量与2、90、120日龄差异极显著(P<0．01),与60日龄差异显著(P<0．05);60日龄表达量与2、 90、120日龄差异显著(P<0．05)。(3)哈萨克羊LPL基因2-60日龄的表达量与IMF含量呈负相关,相关系数为-0．625(P<0．05);新疆细毛羊IMF含量与LPL基因表达量无显著相关性。更多还原

【Abstract】 Male Kazak sheep and Xinjiang fine wool sheep from 2 to 120-day old were selected to investigate the developmental changes and effect on Intramuscular fat (IMF) content of lipoprotein lipase (LPL) mRNA expression in sheep muscle. Six sheep of each breed (only Xinjiang fine wool sheep at 120-day-old) were slaughtered at 2,30,60,90,120 days respectively to sample longissimus dorsi muscle to analyze the IMF content. The total RNA of the samples was extracted to determine the developmental changes of LPL mRNA expression levels by Real time PCR .The results showed that: (1) In male Kazak sheep, the IMF content increased continuously with growing and showed significant difference (P<0.05) between ages, but no difference between ages existed in Xinjiang fine wool sheep (P<0.05). Furthermore, the IMF content of male Kazak sheep was extremely higher (P<0.01) than that of Xinjiang fine wool sheep from 30 to 90 day. (2) LPL mRNA expression level was the highest at 2 day and showed extremely higher than other ages (P<0.01) in male Kazak sheep, it was the lowest at 60 day and then increased. In Xinjiang fine wool sheep, the LPL mRNA was the highest at 30 day (P<0.01), then decreased continuously and to the lowest lever at 90 day; then, the LPL mRNA increased once more. (3) In male Kazak sheep, from 2to 60 day, LPL mRNA expression level was negatively related to IMF content (y = -0.625, P<0.05), and there was no obviously relationship between LPL and the male Xinjiang fine wool sheep.更多还原