【作者】 杨银凤； 火焱； 王秀梅；

【Author】 YANG Yin-feng, HUO Yan, WANG Xiu-mei (Laboratory of Animal Embryo and developmental biology, Inner Mongolia Agriculture University, Huhhot 010018)

【机构】 内蒙古农业大学动物胚胎与发育生物学研究室；

【摘要】 为了检测reBD-1 mRNA在驯鹿体内可能的表达器官,本研究根据已知的reBD-1 cDNA序列设计了一对预计扩增产物为121bp的引物,以驯鹿舌、食管、瘤胃、网胃、皱胃、十二指肠、回肠、结肠、肝、肺、气管、肾、膀胱、睾丸、附睾、心脏、脾脏中提取的总RNA为模板,采用反转录PCR(RT-PCR)技术检测驯鹿的上述器官内reBD-1 mRNA的表达情况。结果显示:reBD-1 mRNA在上述组织器官中均有表达,其中在舌、瘤胃、睾丸中表达最强;在食管、十二指肠、结肠、气管、脾脏中有中等量的表达;在网胃、皱胃、回肠、肝、肺、肾、膀胱、附睾、心脏内的表达较弱。β-防御素reBD-1在驯鹿体内的广泛表达提示reBD-1 有助于驯鹿的先天性宿主防御。更多还原

【Abstract】 In order to examine the organs that express possibly reBD-1 mRNA in a reindeer, we designed a pair of primers, which expected product is 121bp, according the known reBD-1 cDNA sequence. We extracted the total RNA from the tissues of a reindeer’s tongue , esophagus, rumen, reticulum, abomasum, duodenum, ileum, colon, liver, lung, trachea, kidney, bladder, testis, epididymis, heart, spleen and examined the expression of reBD-1 mRNA in above different tissues of the reindeer by RT-PCR. The result indicates that reBD-1 mRNA was expressed in the all organs that was examinated. The best expressed tissues of reBD-1 mRNA was tongue, rumen and testis; middle grade expressed tissues was oesophagus, duodenum, colon, trachea and spleen and in the reticulum, abomasums, ileum, liver, lung, kidney, bladder, epididymis and heart; the expression of reBD-1 mRNA was relatively low. The extensive expression of reBD-1 in reindeers suggests that reBD-1 may play a role in host defense.更多还原