【作者】 董世山； 利凯； 王迎春； 欧德渊； 张建军； 杨玉成； 赵立红； 乔健；

【Author】 DONG Shi-shan LI Kai, WANG Ying-chun, OU De-yuan, ZHANG Jian-jun, YANG Yu-cheng, ZHAO Li-hong, QIAO Jian (College of Veterinary Medicine, China Agricultural University, Beijing 100094; College of Animal Scinece and Technology,Hebei Agricultural University, Baodiing,071001)

【机构】 中国农业大学动物医学院； 河北农业大学动物科技学院；

【摘要】 以Fluo-3／AM为Ca2+指示剂,用LSCM观察缺氧对体外培养的肉鸡心肌细胞内游离Ca2+ 浓度的影响。结果显示,与常氧组比较缺氧显著引起心肌细胞内游离Ca2+升高(常氧99．3 ±13．1;缺氧129．4±24．3,p<0．01),钙通道拮抗剂Verapamil(Ver)和Nifedipine(Nif) 可显著抑制缺氧引起的游离Ca2+升高(缺氧+Ver 100．9±28．2,缺氧+Nif 107．6±27．7)。缺氧是肉鸡腹水综合征(AS)的主要诱因,心肌细胞肥大是AS的主要病理变化。缺氧能够增强心肌细胞的游离Ca2+跨膜转运,结果提示钙的跨膜转运可能与AS的发生发展有关。更多还原

【Abstract】 The purpose of this experiment was to study the efifect of hypoxia on the Ca2+ concentration in broiler’s Cardiac Muscle Cell (CMC) . The concentration of Ca2+ in the CMC was observed by laser scanning confocal microscope (LSCM). The results showed that hypoxia could significantly increase intracellular Ca2+ of CMC (CMC: normal oxgen:99.3±13.1, hypoxia:129.4±24.3,p<0.01.). The Ca2+ antagonist (nifedipine, Nif; verapamil, Ver) can significantly restrain the Ca2+ influx across the cell membrane of CMC treated by hypoxia (CMC: hypoxia + Ver:100.9±28.2, hypoxia+Nif: 107.6±27.7, p<0.01). Hypoxia is the important cause of ascites syndrome (AS), myocardial hypertrophy is the typical pathological change .The results suggest that the transmembrane movement of Ca2+ in the CMC maybe play an important role in the development of AS .更多还原