【作者】 高蔚； 金锋；

【机构】 中国医科大学附属第一医院肿瘤外科；

【摘要】 目的:肿瘤的发生、发展是一个复杂的多步骤的, 经历了多基因、多阶段的,受诸多因素影响的过程。实体肿瘤的生长需要持续的血管生成。针对肿瘤血管生成的研究不断深入,日益成为肿瘤学界研究的热点。目前已被广泛认同和接受的是,肿瘤血管的生成,与多种细胞因子,基因及基因产物有关。当前,信号转导通路与肿瘤血管生成的关系的相关研究已成为一较新的研究热点,但国内有关此方面鲜有报道。本研究采用免疫组织化学染色方法,对60例大肠癌标本间质内微血管标记后定量,对p21ras蛋白表达强度进行分析,探讨其临床病理意义以及两者间的相互关系,以期对信号转导通路与肿瘤血管生成间的关系有更进一步的了解。方法:采用免疫组织化学染色方法,对60例大肠癌标本间质内微血管标记后定量,对p21ras蛋白表达强度进行分析。结果:60例大肠癌组织中p21ras的阳性表达率为70％,MVD为 55．76±17．47。在性别差异、大肠癌分化程度不同的情下, 大肠癌中p21ras、MVD的表达情况无显著性差异(P>0．05)。 p21ras表达为阳性标本的MVD显著(P<0．05)高于p21ras表达为阴性标本的MVD。p21ras的表达与肿瘤大小、淋巴转移情况、肿瘤侵袭深度显著相关(P<0．05);MVD的表达情况与肿瘤大小无显著相关(P>0．05),与淋巴转移情况、肿瘤侵袭深度显著(P<0．05)相关。结论:(1)p21ras的表达与肿瘤大小、淋巴转移情况、肿瘤侵袭深度显著相关; MVD的表达情况与淋巴转移情况、肿瘤侵袭深度显著相关;(2)p21ras表达为阳性标本的MVD显著高于p21 ras表达为阴性标本的MVD,提示癌基因ras突变可导致大肠癌组织中血管生成增强,进一步提示细胞信号转导通路与肿瘤血管生成间存在一定的关系。更多还原

【Abstract】 Objective: By detecting the different level of p21 ras and CD34 in 60 paraffin-embedded tumor tissue of human colorectal carcinoma by immuohistochemistry, we will analyse their clinicopathologic value and relationship. Further to approach the relationship of sig-nal-transduction pathways with tumor angiogenesis in human colorectal carcinoma. Methods: specimens: a group of paraffin-embedded tumor tissue of colorectal carcinoma patients from oncology department of the first clinical hospital of China Medical university were used. 1. All the specimens were investigated by HE. The expression of p21rass, CD34 was investigated by immuohistochemistry.2. Statistics analysis: SPSSIO.O for windows was used. T-test and chi-squared test were the detail ways. Results: In 60 colorectal carcinoma, the positive rate of p21ras 70%, MVD was 55.76 ± 17.47. the expression of p21ras, CD34 was no significant difference in different gender, histological type. MVD with p21ras positive expression was significantly higher than negative. The expression of p21ras was significantly correlated with tumor size , lymph node metastasis and depth of invasion. MVD was not correlated with tumor size, was significantly correlated with lymph node metastasis and depth of invasion. Conclusion: The expression of p21ras was significantly correlated with tumor size, lymph node metastasis and depth of invasion. MVD was significantly correlated with lymph node metastasis and depth of invasion. MVD with p21ras positive expression was significantly higher than negative. 3. Mutation of oncogene ras may enhance angiogenesis in human colorectal carcinoma, further to approach the the relationship of signal-trans-duction pathways with tumor angiogenesis.更多还原