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活性炭快速低压干柱柱层析技术的改进及其在板蓝根分离中的应用
Improvement of Dry-column Flash Chromatography ofactivated charcoal and Its Application of Purification of Isatis tinctoria L.
【作者】 刘雪梅; 秦箐; 林华; 韦建民; 李雪芬; 覃高能; 耿齐统;
【Author】 Liu Xuemei~1 Qin Qing~2 Lin Hua~1 Wei Jianmin~3 Li Xuefen~3 Qin Gaoneng~3 Geng Qitong~3 (1.Postgraduat Student of Guangxi Medical University. 2.Chemistry Department of Guangxi Medical University,Nanning,Guangxi,530021,P.R.China. 3.Student of Guangxi Medical University.)
【机构】 广西医科大学; 广西大学化学化工学院;
【摘要】 对快速低压干柱柱层析进行改进并分离中药板蓝根甲醇部位。以活性炭作为吸附剂,采用快速低压干柱柱层析分离板蓝根的甲醇部位,从板蓝根甲醇部位分离获得2个单点。表明以活性炭作为吸附剂的快速低压干柱柱层析法是一种简单快速的分离方法。
【Abstract】 Improved Dry-column flash chromatography and applied to isolate and purify some fractions from Isatis finctoria L.-Ban Lan Gen-a traditional Chinese medicine.Using Dry-column flash chromatography, activated charcoal as adsorbent,to isolate and purify some fractions from lsatis tinctoria L.Some fractions were isolated from Isatis tinctoria L.and two single spots were performed on the TLC.The Dry-column flash chromatography of activated charcoal is a more simple and faster method in isolation and purification.
【Key words】 Dry-column flash chromatography; activated charcoal; Isatis tinctoria L.; microscale chemical experiment;
- 【会议录名称】 第七届全国微型实验研讨会暨第五届中学微型实验研讨会论文集
- 【会议名称】第七届全国微型实验研讨会暨第五届中学微型实验研讨会
- 【会议时间】2007-07
- 【会议地点】中国广东湛江
- 【分类号】TQ461
- 【主办单位】中国化学会