【作者】 付卫明； 白冬梅； 班睿； 赵学明； 李鑫钢； 徐世民；

【Author】 Fu Weiming, Bai Dongmei, Ban Rui, Zhao Xueming, Li Xingang, Xu Shimin (Department of Biochemical Engineering, School of Chemical Engineering & Technology; National Engineering Research Center for Distillation Technology, Tianjin University, Tianjin, 300072)

【机构】 天津大学化工学院生物工程系； 天津大学精馏技术国家工程研究中心；

【摘要】 采用反相高效液相色谱法测定了生孢乳杆菌发酵液中的乳酸。在Eclipse XDB-C8, (4．6 mm i．d．× 150 mm,5 μm)色谱柱上,以 0．005 mol／L硫酸(pH值2．5)溶液作流动相,流速为1 mL／min,以示差折光检测器检测信号,峰面积(Y)与乳酸的质量浓度(x,g/L)的线性相关方程为Y=292．08x+4．696 7,线性相关系数r为0．999 9,线性范围为0．75-9．0g／L,检测限为0．2μg(3倍信噪比)。结果表明,实验方法简便、快速、可靠,精密度为0．22％(m=5),回收率99％以上。更多还原

【Abstract】 A method for determining lactic acid in fermentation broth of Sporolactobacillus inulinus by RP -HPLC was described. The operating conditions were Eclipse XDB - C8 column (4.6 mm i. d. × 150 mm, 5 μm) at room temperature, 0. 005 mol/L sulfic acid solution (pH 2. 5) as mobile phase with flow rate of 1.0 mL/min and the detector is refractive index detector, the retention time of lactic acid is 2.243min. The linear correlation equation was Y = 292. 08x + 4. 696 7 ( r = 0. 999 9), the linear range is 0. 75 -9. 0g/L, and the detection limit is 0. 2 μg (signal to noise ratio is 3). This method is simple, quick, accurate and reproducible, the result is not affected by other components of fermentation broth. The relative standard deviation (RSD, n=5) was 0.22% , and the average recovery was above 99% .更多还原