【作者】 姚鹏； 黄杰； 郭毅； 赵爱杰； 常津；

【Author】 Peng Yao Jie Huang Yi Guo Aijie Zhao Jin Chang* Institute of Nanobiotechnology, School of Materials Science and EngineeringTianjin University, Tianjin 300072, P.R.China

【机构】 天津大学材料学院纳米生物技术研究所；

【摘要】 本文以O-羧甲基壳聚糖(O-CMC)为原料,制备出具有超顺磁特性的纳米载体粒子,并首次将跨膜肽(Tat),人转铁蛋白(Transferrin)与其结合,选择甲氨喋呤(MTX)为模型药物,构建成平均粒径75nm左右的具有抗癌和跨膜功能的复合靶向纳米载体系统(MTX-TF-TAT-O-MNPs)。应用透射扫描电镜(TEM),原子力显微镜(AFM),VSM磁分析仪及X射线衍射仪表征该纳米载体系统的形貌,粒径,磁性能以及晶形结构。最后我们研究了磁性纳米载药粒子的穿透性,通过单光子发射型计算机断层显像仪(SPECT)证明了该粒子可以成功的跨过血脑屏障(BBB)。更多还原

【Abstract】 This paper describes a new formulation of magnetic nanoparticles coated by a novel polymer matrix----O-Carboxylmethylated Chitosan (O-CMC) as a drug/gene carrier. The O-CMC magnetic nanoparticles were derivatized with a peptide sequence from the HIV-tat protein and transferrin to improve the translocational property and cellar uptake of the nanoparticles. To evaluate the O-MNPs-Tat-Tf as a drug carrier, Methotrexate (MTX) was incorporated as a model drug and MTX-loaded O-MNPs-Tat-Tf with an average diameter of 75nm were prepared and characterized by TEM, AFM, VSM and XRD. We also studied the ability of O-MNPs-Tat-Tf to cross the BBB in rats by single photon emission computed tomography (SPECT).更多还原