【作者】 刘刚； 汤晴； 张捷； 王海涛； 姜韧； 谭生建；

【Author】 LIU Gang, TANG Qing, ZHANG Jie ,WANG Hai-Tao, JIANG Ren and TAN Sheng-Jian ( Department of Pharmacy, Hospital 306 PLA Beijing 100101)

【机构】 中国人民解放军第306医院药学部；

【摘要】 目的:建立高效液相色谱法同时测定氯霉素甲硝唑洗鼻剂中中氯霉素、甲硝唑含量测定方法及氯霉素的有关物质氯霉素二醇物、对硝基苯甲醛的检查方法。方法:采用高效液相色谱法。Intersil C18分析色谱柱(4.6mmID×250mm,粒径5μm),流动相:甲醇―水-三乙胺(480:520:7,含1%磷酸二氢钠,0.2%庚烷磺酸钠,磷酸调pH值为3.0),流速1.0mL·min-1,检测波长270nm。结果:甲硝唑、氯霉素二醇物、氯霉素和对硝基苯甲醛的理论板数分别为4700、4500、4400、1500。回归方程分别为:y=424145.7+19156100.5X(r=0.9999),y=39817070.9x+1373589.3(r=0.9998),y=65390024.2x-5660.6(r=0.9999),y=83051977.5x-284191.7(r=0.9998);线性范围分别为0.4230μg～2.115μg,0.4500μg～2.250μg,0.0330μg～0.1650μg,0.02220μg～0.1110μg;平均回收率(±RSD)分别为99.5%(±1.4),98.7%(±1.3),102.3%(±1.8%),96.3%(±1.9);最低检出限分别约为0.0002,0.0002,0.0002和0.003μg。结论:用高效液相色谱法同时测定氯霉素甲硝唑洗鼻剂中甲硝唑、氯霉素二醇物、氯霉素、对硝基苯甲醛的含量,操作简便,结果准确。更多还原

【Abstract】 Objective: A quantitative method was established for the determination of Chloramphenicol ,Metronidazole , 2-Amino-1-(4-nitrophenyl)propane-1,3-diol and 4-Nitrobenzaldehyde in Collunarium by means of HPLC. Method: The chromatographic conditions includes Intersil C18 column and mobile phase consist of methanol-water-triethylamine (480:520:7,containing 1% NaH2PO4,0.2% 1-heptanesulfonate, sodium salt, to regulate pH to 3.0 with phosphoric acid) .The flow rate was 1.0 mL·min-1 . The detective wavelength was 270nm performed at room temperature . Results: The number of theoretical plates caculated by metronidazole, 2-Amino-1-(4-nitrophenyl)propane-1,3-diol, chloramphenicol and 4-Nitrobenzaldehyde peak were 4700,4500 ,4400 and 1500 respectively; the regression equation was y = 424145.7 +19156100.5 x(r = 0.9999 ), y = 39817070.9x + 1373589.3(r = 0.9998 ),y = 65390024.2 x - 5660.6(r = 0.9999), y = 83051977.5 x - 284191.7 (r = 0.9998) respectively;the linear range was 0.4230μg～ 2.115 μg , 0.4500μg～ 2.250 μg, 0.0330μg ～ 0.1650μg, 0.02220μg～ 0.1110 μg respectively; the average recovery(±RSD)was 99.5%(± 1.4 %),98.7%(±1.3%),102.3%(±1.8 %),96.3%(±1.9 %)respectively;the measurable lowest limit 0.0002, 0.0002, 0.0002,0.003μg respectively. Conclusion: The method is rapid, accurate and reliable.更多还原