【作者】 陶永辉； 张莲芬； 金坚；

【Author】 TAO Yong-hui, ZHANG Liang-feng, JIN Jian (Department of Pharmaceupical and Molecular Biotechnology, School of Biotechnology, Southern Yangtze University, Wuxi, PR China 214036)

【机构】 江南大学生物工程学院生物制药系；

【摘要】 采用99Tcm-rhAS,在S180肿瘤和Matrigel模型上分别进行了显像及放射自显影的研究,以求明确AS的作用特异性。结果发现S180肿瘤及Matrigel模型对99Tcm-rhAS特异性摄取,表明AS可与新生血管特异性结合,其抑制肿瘤的作用是基于靶向血管内皮细胞的。采用亲和甑别技术对重组人血管抑素(Recombinant human angiostatin,rhAS)结合蛋白进行了纯化,质谱测序并与蛋白质数据库进行了比较。结果发现有多个rhAS结合蛋白,采用蛋白跨膜预测软件Tmpred对其进行了分析,采用分子对接软件DOCK计算了其与AS(K1-3)的结合情况。并以人血管内皮细胞(HMEC-1)为模型对可能的结合蛋白Tubulin的作用进行了分析。更多还原

【Abstract】 To study the specificity of action of angiostatin on vascular endothelial cell, the effect of 99Tcm-angiostatin in vivo were observed on the model of mice bearing S180 tumor and Matrigel by imaging and radioautography. The results indicated that the S180 tumor and Matrigel all can specified uptake 99Tcm-angiostatin. The role of angiostatin inhibiting tumor is found on the basis of targeting novel vascular endothelial cell. The binding proteins of recombinant human angiostatin(rhAS) on HMEC-1 cell were isolated by affinity chromatography, and the binding proteins were sequenced by Mass Spectrometry, then compared with IPI Human Protein Bank. The results indicated that there are several binding proteins of rhAS, and then the structure of the binding proteins of rhAS were analyzed with Tmpred software, the binding between AS(K1-3) and tubulinα+β,Actin,ATP synthase βwas analyzed with DOCK software. Furthermore, the effect of rhAS and anti-Tubulin α/β on HMEC-1 cell line was measured to analyze the action of tubulin.更多还原