【作者】 夏广萍； 韩英梅； 刘鹏；

【Author】 XIA Guang-ping,HAN Ying-mei,LIU Peng(Tianjin Institute of Pharmaceutical Research,Tianjin300193,China)

【机构】 天津药物研究院；

【摘要】 目的建立黄芪药材指纹图谱的研究方法。方法采用高效液相色谱-蒸发光散射检测法(HPLC-ELSD),Alltech C18色谱柱;流动相:乙腈-水(32:68);流速:1.0mL·min-1;检测器:ELSD2000。结果该方法具有良好的稳定性和重复性,通过对10批黄芪药材的测定,建立了黄芪药材的指纹图谱。结论利用黄芪药材指纹图谱分析法可较全面的反应内蒙固阳产黄芪药材的内在质量,可用于当地产黄芪药材的质量控制。更多还原

【Abstract】 OBJECTIVE To determine the fingerprint of Radix Astragali. METHODS The fingerprint of Radix Astragali was established by HPLC-ELSD. The chromatographic separation was perfermed on an Alltech C18 column. The mobile phase consisted of 32% acetontrile and 68% water. The flow rate was 1.0mL·min-1.The detection was ELSD2000. RESULTS The method is simple and accurate with good reproducibility.The HPLC fingerprint was established through the results of 10 batches of Radix Astragali. CONCLUSION The HPLC fingerprint method can be used for quality control of Radix Astragali from Guyang.更多还原