【作者】 张英鸽； 王琛； 赵德禄； 白春礼； 林樟； 田芳； 苏明；

【Author】 ZHANG Ying-ge~1 ZHAO De-lu~1 BAI Chun-li~2 WANG Chen~2 SU Ming~2 LIN Zhang~2 TIAN Fang~2 (1 Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences,Beijing 100850,China.) (2 Institute of Chemistry,Academia Sinica,Beijing 100080,China.)

【机构】 军事医学科学院毒物药物研究所； 中国科学院化学研究所；

【摘要】 本文研究了用原子力显微技术对乙酰胆碱酯酶进行成像的方法。在新鲜裂解的云母表面镀一层金膜,金膜与疏基丙酸(MPA)的疏基形成 S-Au 键。使 MPA 的游离羧基再通过碳二亚胺反应与 AChE 的碱性氨基酸的氮基形成肽键连接,AChE 因而被固定于支持物表面。这样固定的 AChE 可获得清晰成像而不脱落。更多还原

【Abstract】 Method of imaging acetylcholinesterase(ACHE)with atomic force microscopy has been studied.A gold film was evaporated onto the fresh surface of mica.By immersing the gold film in the mereaptopropionic acid(MPA)solution,Au-S bond was formed between Au and the hydrosulfide group.The carboxyl group of MPA was activated by N-ethyl-N’-(3-dimethylaminopropyl)carbodiimide(EDC)in the solution of EDC and then was allowed to react with AChE and thus peptide bond between the carboxyl group of MPA and the free amino group of the amino acid in AChE molecules.Clear image of AChE immobilized in this way could be obtained by atomic force microscopy.更多还原