【作者】 张晶； 侯本祥； 张琛； 刘红岩；

【Author】 ZHANG Jing,HOU Ben-xiang, ZHANG Chen,LIU Hong-yan. (Department of Endodontics, College of Stomatology, Capital University of Medical Sciences. Beijing 100050, China)

【机构】 首都医科大学口腔医学院牙体牙髓科；

【摘要】 目的:对4种第7代牙本质粘接系统的体外细胞毒性进行对比研究,为临床应用提供参考依据。方法:采用体外细胞培养技术和四甲基偶氮唑盐(MTT)比色法,观察4种第7代牙本质粘接系统Ciearfil tri-S Bond、G-Bond、i-Bond和XenoⅣ材料浸提液对L929小鼠成纤维细胞生长的影响,测定各组吸光度值(OD值),计算L929小鼠成纤维细胞的相对增殖率,用5级毒性分类法评级,并进行统计分析。结果:XenoⅣ组的OD值显著低于其他各组的OD值。结论:Clearfil tri-S Bond、G-Bond和i-Bond 组的体外细胞毒性较弱,XenoⅣ组的体外细胞毒性较高。更多还原

【Abstract】 OBJECTIVE: Comparison of cytotoxicity in vitro of four kinds of dentin adhesive agents(Clearfil tri-S Bond、G-Bond、i-Bond、XenoⅣ)from different kinds on L929 mouse fibroblast was observed. METHOD: Using tetrazolium-based colorimetric assay in vitro, to determine light absorption value of every hole with enzyme linked immunity instrument after2d ,4d and 6d of contact of evaluate them by five degree tests and statistic analysis for cytotoxicity. RESULTS: Cytotoxicity of these adhesives ranges from 1-3 of them existing apparent differences. CONCLUSION: Among of them the cytotoxicity of XenoⅣ was the highest.更多还原