【作者】 张志光； 张谦； 何一青； 陈诗书；

【Author】 Zhang Zhiguang Zhang Qian He Yiqing Chen Shishu Department of Oral and Maxillofacial Sugery, College of Stomatology, Sun Yat-Sen University of Medical Science

【机构】 中山医科大学口腔医学院； 上海第二医科大学人类基因治疗研究中心；

【摘要】 <正>目的:观察细胞因子基因修饰的人口腔鳞癌细胞致瘤性,探讨细胞因子基因转染的抑瘤作用。方法:以携带TNF-α,IFN-γ和IL-2 cDNA的逆转录病毒为载体转染人口腔鳞癌细胞株Tca8113, 获得子株Tca-TNF、Tca-IFN和Tca-IL-2。各以2×106个细胞接种BALB／c裸小鼠右腋皮下,每组小鼠10只。每周以卡尺测定成瘤的最大长径和宽径,计算体积,5周后处死动物,剥离肿瘤称重。更多还原

【Abstract】 Objective: To observe the tumorigenicity of the cytokine genes modified oral squmous cell carcinoma cells. Methods: There derivates of Tca-8113, and oral squsmous carcinoma cell line, modified by retroviral-mediated TNF-α, IFN γ and IL-2 cDNA respectively, were xenografted into BALB/c nude mice, on 2×106 cells each. Results: The growth of the modified cells in nude mice was slower than their parental cells. The average weights of the tumors formed by Tca-TNF, Tca-IFN and Tca-IL-2 were inferior to that of Tca-8113 (P<0.05). Conclusion: The tumorigenicity of cytokine gene modified cells decreased significantly compared with that of wild-type and mock infected Tca8113 cells. It suggested that inhibition of the tumorgenicity attribute to cytokines.更多还原