【作者】 张红梅； 张红伟； 刘小红； 郑祖平； 王富荣； 杨书成； 罗绮； 谭振波；

【Author】 ZHANG Hong-mei WANG Fu-rong YANG Shu-cheng LUO Qi Maize Research Institute,Shanxi Academy of Agricultural Science,Shanxi Xinzhou 034000,China; ZHANG Hong-wei TAN Zhen-bo Beijing DBN Institute of Agricultural Sci & Tech,Beijing 100085,China; LIU Xiao-hong ZHENG Zu-ping Maize Research Institute,Sichuan Agricultural University,Sichuan Ya’an 625014,China

【机构】 山西省农科院玉米研究所； 北京大北农农业科技研究院； 四川农业大学玉米研究所；

【摘要】 以黄早四(高抗玉米矮花叶病)和 Mo17(高感玉米矮花叶病)为亲本建立了包含239个重组自交系的群体,利用该群体构建了包含101个 SSR 标记的遗传连锁图,这101个 SSR 标记分布在10个连锁群上,覆盖玉米基因组1422.7cM,标记间的平均距离为 15.6cM。通过病毒人工接种鉴定,评价了这239个 RIL 系和亲本对 MDMV 病毒 B 株系的抗性反应。采用复合区间作图法对玉米矮花叶病抗性 QIL 进行了分析,结果在第6染色体上定位了2个主效 QTL(Qmdmvla 和 Qmdmvlb),分别与分子标记 bnlg161、ylssr 连锁,能解释表型方差的56.5%和76.8%。这两个来自于黄早四的 QTL 的加性效应可分别使病情指数下降30.7%和37.3%。更多还原

【Abstract】 The MDMV-resistant inbred line Huangzao 4 and the highly susceptible inbred line Mo17 were used to develop a population consisted of 239 RILs(Recombinant Inbred Lines).A SSR linkage map was constructed with 101 SSR loci distributed on 10 linkage groups,coveting 1422.7cM with average interval length 15.6cM.239 RILs and their parental lines Mo17 and Huangzao4 were evaluated for their resistance to MDMV B strain under artificial inoculation.With the method of composite interval mapping,two major QTLs for resistance to MDMV strain B were identified and lo- cated on chromosome 6,linked with trinkets bnlg161 and ylssr,were identified with major effects conferring resistance to MDMV,which can explain 56.5% and 76.8% of the phenotypic variance,respectively.更多还原