【作者】 刘传光； 江奕君； 毛兴学； 高云； 刘军； 林青山； 李晓芳；

【Author】 LIU Chuan-guang JIANG Yi-jun MAO Xin-xue GAO Yun LIU Jun LIN Qing-shan LI Xiao-fang Rice Research Institute,Guangdong Academy of Agricltural Sciences,Key Laboratory of Crop Genetics and Breeding,Ministry of Agriculture,Guangdong Guangzhou 510640,China

【机构】 广东省农业科学院水稻研究所农业部作物遗传育种重点开放实验室；

【摘要】 选用7对 SSR 引物对7个水稻×玉米远缘后代不育株系及其双亲进行 SSR 分析。与母本扩增带型相比,共扩增出18条特异带,即表现为多态性。在检出的18条特异带中,13条的带型与父本相同,另5条的带型不同于双亲,即为变异带型。7个参试后代不育株系都检测到了 SSR 多态性标记,但各株系间检出多态性位点的数量存在差异,最多的一个株系 ys-2同时检测到3个多态性标记。试验结果表明通过远缘杂交技术很可能将玉米的某些 DNA 片段成功地导入了水稻两用核不育系,表明本研究在籼稻两用核不育系的改良上取得了初步的成果。更多还原

【Abstract】 Seven SSR primers were used to identify 7 rice and maize wide hybrid posterity Indica rice PTGMS lines.In comparison with female par- ents,18 polymorphic bands are specific bands,namely SSR polymorphism.In the specific bands,13 bands were identical with that of the male parent, and the other 5 bands,which were different from that of the parents,were variant bands.All of the posterity indica rice PTGMS lines were detected of SSR polymorphism,but the quantity of sites being detected is variant between the lines.The most one is ys-2,in which three SSR polymorphic sites were got.It was preliminarily proved that some DNA segments of maize might have been transferred into rice PTGMS lines by rice and maize wide hy- brid,and the improvement of Indica rice PTGMS lines had been succeeded preliminarily.更多还原