【作者】 周华仕； 娄金萍； 陈钧强； 王洁宏； 钟惠仙； 张幸； 洪长福； 俞少勇；

【Author】 Zhou Hua-shi,Lou Jin-ping,Chen Jun-qiang,et al (Institute of Hygiene, Zhejiang Academy of Medical Sciences,Hangzhou,310013)

【机构】 浙江省医学科学院卫生学研究所；

【摘要】 目的研究蔺草染土粉尘的致尘肺作用。方法 SD雄性大鼠以非暴露式气管内染尘(50mg/只),于染尘后3、6、12个月,取大鼠肺和肺淋巴结作病理和电镜观察,测定肺组织胶原蛋白和血清铜蓝蛋白(CP)含量。结果染尘后12个月,蔺草染土尘组大鼠肺湿重(2．69±0．22)g,高于对照组[(1．87±0．25)g]和TiO2组[(2．25±0．26)g],但低于SiO2组[(11．41±1．63)g];蔺草染土尘组大鼠肺干重(0．47±0．03)g,高于对照组[(0．32±0．03)g]和TiO2组[(0．41± 0．08)g];但低于SiO2组[(2．06±0．28)g];蔺草染土尘组大鼠肺胶原蛋白含量[(103．08±14．79)mg]高于对照组 [(75．96±13．91)mg]和TiO2组[(85．84±17．62)mg],但低于SiO2组[(497．50±100．80)mg];蔺草染土尘组大鼠血清CP含量为(18．03±1．87)U/L,高于对照组[(15．05±2．24)U/L]和TiO2组[(16．92±1．67)U/L],但低于SiO2组[(25．37±3．58)U/ L]。以上各指标各组间的差异均有显著性(P<0．05或P<0．01)。蔺草染土尘组大鼠病理观察可见肺巨噬细胞肺泡炎、Ⅱ型肺泡上皮细胞增多及肺泡间隔增宽,有1—2级矽结节和少量胶原纤维性变。电镜下可见,蔺草染土尘组大鼠肺有初级和次级溶酶体及髓鞘样吞噬残体,肺泡隔Ⅱ型肺泡上皮细胞增多和胶原纤维略有增多,但比SiO2 所致病变轻。结论蔺草染土粉尘对大鼠肺部有轻度致纤维化作用。更多还原

【Abstract】 Objective To investigate the fibrogenetic effects induced by rushmat dust in rats. Methods SD rats were treated with 50 mg of rushmat dust per rat by intratracheal instillation, sacrificed 3,6,and 12 months respectively after exposure.The lung tissue and lung limphnode were taken out for pathological and electron microscopic examination.The content of collagen and ceruloplasmin (CP)in lung tissues were also determined.Results After treatment for 12 months, fresh wet lung weight in rushmat dust group [(2.69+0.22)g]was higher than those in saline group [(1.87±0.25)g],TiO2 group [(2.25±0.26)g],but lower than that in SiO2 group [(11.41±1.63)g];dry lung weight in rushmat dust group [(0.47±0.03)g]was higher than those in saline group [(0.32±0.03) g],TiO2 group[(0.41±0.08)g],but lower than that in SiO2 group[(2.06±0.28)g];lung collagen content in rusnrmat dust group [(103.08±14.79)mg]was higher than those in saline group [(75.96±13.91) mg],TiO2 group [(85.84±17.62)mg]but lower than that in SiO2 group [(497.50±100.80)mg];CP content in rustrmat dust group[(18.03±1.87)U/L]was higher than those in saline goup[(15.05±2.24) U/L],TiO2 group[(16.92±1.67)U/L],but lower than that in SiO2 group[(25.37±3.58)U/L],P<0.05 or P<0.01.Pathological examination showed lung macrophage alveolitis,broadening of alveolar interval, one to two grade of silicotic nodes and increased amount of type II epithelial cell in alveola as well as slight collagenous fibrosis in lung tissue of rush mat dust group.Under electron microscope, primary and secondary lysosome andmedullary sheathlike phagocytic residual body were found in lung tissue of rushmat dust group,meanwhile the amount of type II alveolar epithelial cell and collagen fiber were slightly increased but these changes were less than those of quartz group. Conclusion The rushrmat dusts have slight pulmnonary fibrogenetic effect on rat.更多还原