【作者】 欧阳彤； 陈胜； 汪凤珍；

【Author】 Ouyang Tong Chen Sheng Wang Feng-zhen The Research Institute of Subtropical Forestry CAF Fuyang Zhejiang 311400 China

【机构】 中国林科院亚热带林业研究所；

【摘要】 本文研究了基本培养基(改良MS、改良KC、VW及自制ZW系列)、植物激素(6-BA、NAA、IBA、 KT)、培养条件(光照、有机添加物、糖分)等相关因子对文心兰组织培养工厂化育苗中瓶苗质量的影响。结果表明:1．当6-BA 0．1-2mg／1,NAAO．1-0．5mg／1时,有助于诱导拟圆球茎形成与增殖;2．苗化及壮苗培养阶段低浓度植物激素,有助于降低畸形率,高盐浓度矿质营养有较高的致畸率;3．文心兰可以在无糖培养基中自养生长;4．有机添加物,有助于文心兰瓶苗健化;5．添加NAA、IBA 0．1-0．5mg／1 时,均可诱导生根;6．多代培养后,培养条件要随之作相应调整,以复壮瓶苗质量;7．基本培养基对文心兰组培工厂化育苗有显著影响,自制培养基ZW系列更适合于文心兰组培快繁生产:更多还原

【Abstract】 This paper study the effect on the seedling quality of Oncidium by correlation factors such as minimal medium (improve MS improve KC VW and ZW series which from ourselves etc. ). phytohormone (6-BA NAA. IBA KT) cultural condition (illumination aero additive sugar etc) and so on during industrial propagation by tissue culture. The result shows that: 1. It is helpful to induce and increase the protocorm when the concentration of 6-BA is between 0.1 and 2mg/l and NAA :is between 0.1 and 0. 5mg/l. 2. The low concentration of auxin is good for reducing the malformation during protocorm differentiation and seedling growth and high concentration of mineral nutrition is easy to induce malformation of seedling; 3. Oncidium is autotrophic without sugar in the medium; 4. Organic additive is benefit to improve the quality of test-tube plantlet; 5. It is good for root emerge when medium are added NAA’ IBA both concentration between 0.1 and 0. 5mg/l; 6: It is necessary to regulate the cultural conditions after many time regeneration for invigorate test-tube plantlet quality; 7: minimal mediums have notable effects on the industrial propagation of Oncidium by tissue culture and the ZW series mediums which made by ourselves are more suitable to Oncidium propagation by tissue culture.更多还原