【作者】 劳杰； 熊良俭； 赵新； 顾玉东； 李继峰；

【Author】 LAO Jie HUNG Leungkim ZHAO Xin et al.Department of Hand Surgery Huashan Hospital Fudan University Shanghai 200040 China

【机构】 复旦大学华山医院手外科；

【摘要】 目的:了解华勒变性过程中雪旺细胞的形态学变化。方法:选用42只SD大鼠, 在腋部切断正中神经和尺神经,以远侧段作为预变神经,术后当天3 d和1、2、3、4、8周(1- 7组)取材,通过雪旺细胞培养S-100蛋白标记及电子显微镜技术观察雪旺细胞数量和髓鞘厚度。结果:神经被切断后,雪旺细胞的数量开始增加,预变后1周达到高峰,约为3．52±27×107个／ml神经被切断后,髓鞘的厚度也相应增加,预变后1周达到最厚,约为231．2±8．9nm,随后缓慢变薄。结论:在华勒变性过程中雪旺细胞的数量和髓鞘厚度随变性时间的增加而发生变化。更多还原

【Abstract】 Objective To study the morphological changes of the Schwann cells within the prede-generated nerve. Methods The ulanr and the median nerves were cut bilaterally at the axillary level in 42 SD rats. A nerve segment of 1 cm in length was harvested from the distal segment of each of the cut nerves right after the operation 3 days 1, 2, 3, 4 and 8 weeks postoperatively group 1-7. The methods of Schwann cells culture and S-100 protein standing were used to study the temporal changes of the number of the Schwann cells and the thickness of myelin of the predegenerated nerve were studied under electromicro-scope. Results The number of Schwann cells increased after nerve transection and reached a peak of 3.52±0.27×107/ml in one week.The thickness of myelin also increased after nerve transaction and was thickest at about 231.2±8.9 nm in one week. The myelin thickness slowly decreased after this time. Conclusion The number of the Schwann cells and the thickness of myelin of the predegenerated nerve were changed with time.更多还原