【作者】 张淑萍； 梁燕； 邓常青；

【机构】 湖南中医药大学中西医结合学院；

【摘要】 目的研究补阳还五汤及其有效部位生物碱、苷对脑缺血再灌注后Caspase-1、3、8 表达的影响。方法采用颈内动脉线栓法建立大鼠MACO再灌注模型,以逆转录-聚合酶链反应(RT-PCR)测定缺血脑组织Caspase-1、3、8 mRNA的表达。结果脑缺血2h再灌注22h后,模型组Caspase-1、3、8表达较假手术组均显著增强(P<0．05)。生物碱组、苷组 caspaLse-1表达显著低于模型组(P<0．05);补阳还五汤组、生物碱组、苷组Caspase-3与模型组比较均显著降低(P<0．05)．生物碱组Caspase-8表达较模型组明显降低(P<0．05)。结论补阳还五汤及其有效部位通过抑制缺血后脑组织Caspase-1、3、8的表达,从而有可能对抗脑缺血再灌注后神经细胞凋亡,保护缺血脑组织。更多还原

【Abstract】 Objective: To investigate the effects of Buyang Huanwu Decoction (BHD) and two kinds of fractions extracted from BHD on the expression of Caspase-1、3、8 mRNA after cerebral ischemia reperfusion in rats. Methods; The model of cerebral ischemia-reperfusion was established by middle cerebral artery occlusion artery in rats. The expression of Caspase-1、 3、 8mRNA in cerebral ischemic tissues was assessed by reversetranscription-polymerase chain reaction(RT-PCR). Results: The expression of Caspase-1、 3、 8mRNA in cerebral tissues was significantly increased after 24 hours of reperfusion following 2 hours of cerebral ischemia in model group (P<0.05).Compared with model group ,the expression of Caspase-l was decreased remarkably in Alkaloid group and Glycoside group (P<0.05). BHD、 Alkaloid and Glycoside can reduce the expression of Caspase-3 significantly (P<0.05) .The expression of Caspase-8 in Alkaloid group was decreased compared with that in model group (P<0.05) . Conclusion: BHD、 Alkaloid and Glycoside can inhibit the expression of Caspase-1 、 3、 8 mRNA after cerebral ischemia reperfusion injury, then antagonize the nervous cell apoptosis and protect the cerebral ischemic tissues.更多还原