【作者】 杜玉珍； 高锋； 童建；

【Author】 (Du Yuzhen,Gao Feng,Tong jian. Center of Laboratory Medicine,the Sixth Hospital of Shanghai, Shanghai Jiaotong University,Shanghai,China,200233. Department of Toxicology,School of Radiation Medicine and Public Health,Soochow University of Suzhou,Suzhou,215123)

【机构】 上海市第六人民医院； 苏州大学放射医学与公共卫生学院；

【摘要】 目的探讨生物钟基因Clock及相关褪黑素受体基因(mt1和mt2)在外周血淋巴细胞中的表达规律,以发现外周血淋巴细胞中是否存在分子生物时钟。方法以不同光制(全黑暗和光照-黑暗交替光制)条件下饲养的 SD大鼠为研究对象,收集不同时点的外周血淋巴细胞,采用半定量RT-PCR法检测钟基因Clock及相关基因mt1 和mt2在转录本水平上的表达丰度,并用余弦软件分析各基因表达的昼夜节律特征。结果发现在光照-黑暗交替光制(LD12:12)下,Clock, mt1和mt2在大鼠外周血淋巴细胞中都具有昼夜节律性表达,其峰值位相分別 为ZT21、ZT8和ZT6;在全黑暗光制(DD)下,这种节律性表达现象仍然存在,即它们的表达节律是内源性的, 其峰值位相分别位于CT7、CT9、CT11。结论外周血淋巴细胞中可能存在受褪黑素调节的分子生物钟。更多还原

【Abstract】 Objective To explore the expression patterns of Clock and clock-related genes in the peripheral lymphocytes,and to infer if a molecular circadian clock might be existed.Methods The circadian expression patterns of the Clock and melatonin receptor genes (mt1,mt2) in the peripheral lymphocytes were determined by the method of semi-quantitative RT-PCR,and then analyzed by cosine software. Results Circadian expressions of the Clock and melatonin receptor genes (mt1,mt2) were found in the light-dark regime of LD(12:12), with the peak phase at ZT21,ZT8 and ZT6,respectively;the circadian expressions were observed in the constant dark regime(DD) too, with the peak phase at CT7, CT9 and CT11,respectively.That is,the circadian expression of these genes is endogenetic.Conclusion A molecular circadian clock regulated by melatonin might be existed in the peripheral lymphocytes of rats.更多还原