Salvia Miltirrhiza Bunge and Curcuma Longa Linn extractions reduce the phosphorylated extracellular signal regulated kinase1/2 expression level in rat hepatic stellate cells T6

【Author】 CHENG Yang, PING Jian, TAN Yinzi,CHEN Gaofen Institute of liver diseases,Shanghai University of TCM,Shanghai, 201203 Institute of liver diseases,Shanghai University of TCM

【摘要】 <正>Objective:To study the effect of Salvia Miltirrhiza Bunge extraction (Salvianolic acid B) and Curcuma Longa Linn extraction (Curcumin) on the proliferation, activation and production of extracellular matrix in rat hepatic stellate cells, and the relation between the effect and the extracellular signal regulated kinase expression level and its phosphorylation.Methods: The rat T6 hepatic stellate cells were cultured and treated by Salvianolic acid B or Curcumin according to the experimental protocols. The inhibition effect on the cell proliferation was determined by 3- (4, 5-dimthy-2-2thiazoly) 2, 5-dipheny-2H-tetrazoliun bromide colorimetry. The cells were harvested and lysed to extract the total cellular proteins. Proteins were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and the expression levels of a smooth actin, type I collagen, and extracellular signal-regulated kinase after drug treatments were determined by Western blot.Results: Salvianolic acid B and Curcumin inhibited the proliferation of rat hepatic stellate cells in dose-dependent fashion, reduced the expression level of α-smooth muscle actin significantly (P<0.01) . Curcumin reduced the expression of type I collagen significantly (P0.05) . Both of Salvianolic acid B and Curcumin had no significant effect on the expression level of extracellular signal-regulated kinase, but could significantly down-regulate phosphorylated extracellular signal-regulated kinase expression level (P0.05 vs. P0.01, respectively) .Conclusion: Salvianolic acid B and Curcumin could significantly inhibit the proliferation, activation and production of extracellular matrix in rat hepatic stellate cells, and the effect was associated with the reduction of phosphorylated extracellular signal regulated kinase 1/2 expression level. The drug or drug compound with multi-effect-pathways and multi-effect-targets may have better anti-hepatic fibrosis effect than a single drug alone.更多还原

【Abstract】 Objective:To study the effect of Salvia Miltirrhiza Bunge extraction (Salvianolic acid B) and Curcuma Longa Linn extraction (Curcumin) on the proliferation, activation and production of extracellular matrix in rat hepatic stellate cells, and the relation between the effect and the extracellular signal regulated kinase expression level and its phosphorylation.Methods: The rat T6 hepatic stellate cells were cultured and treated by Salvianolic acid B or Curcumin according to the experimental protocols. The inhibition effect on the cell proliferation was determined by 3- (4, 5-dimthy-2-2thiazoly) 2, 5-dipheny-2H-tetrazoliun bromide colorimetry. The cells were harvested and lysed to extract the total cellular proteins. Proteins were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and the expression levels of a smooth actin, type I collagen, and extracellular signal-regulated kinase after drug treatments were determined by Western blot.Results: Salvianolic acid B and Curcumin inhibited the proliferation of rat hepatic stellate cells in dose-dependent fashion, reduced the expression level of α-smooth muscle actin significantly (P<0.01) . Curcumin reduced the expression of type I collagen significantly (P0.05) . Both of Salvianolic acid B and Curcumin had no significant effect on the expression level of extracellular signal-regulated kinase, but could significantly down-regulate phosphorylated extracellular signal-regulated kinase expression level (P0.05 vs. P0.01, respectively) .Conclusion: Salvianolic acid B and Curcumin could significantly inhibit the proliferation, activation and production of extracellular matrix in rat hepatic stellate cells, and the effect was associated with the reduction of phosphorylated extracellular signal regulated kinase 1/2 expression level. The drug or drug compound with multi-effect-pathways and multi-effect-targets may have better anti-hepatic fibrosis effect than a single drug alone.更多还原