【作者】 田兆丰； 裘季燕； 刘伟成； 李永丹；

【Author】 TIAN Zhao-feng, QIU Ji-yan, LIU Wei-cheng, LI Yong-dan (Institute of Plant & Environment Protection, Beijing Academy of Agriculture & Forestry Sciences, Beijing 100089, China;Department of Plant Protection,China Agriculture University,Beijing 100094,China)

【机构】 北京市农林科学院植保环保所； 中国农业大学植物保护系；

【摘要】 采用黄瓜花叶病毒(CMV)亚组Ⅰ株系Fny-CMV及亚组Ⅱ株系Ls-CMV的RNA2的特定序列片段的cDNA克隆,体外转录,同时掺入32P标记制备负链RNA探针,再与纯化的甜椒上的CMV中国分离物的RNA进行杂交,检测其与探针之间的同源性。共检测样品分离物3份。试验结果表明:河南新乡和北京密云的CMV甜椒分离物与Fny-CMV的核苷酸有高度同源性,隶属于Fny-CMV为代表的亚组Ⅰ株系。来自福建的样品与亚组Ⅱ的Ls-CMV株系有高度同源性,隶属于 CMV亚组Ⅱ株系。本试验同时利用源于我国CMV亚组Ⅰ的K株系的RNA2两个EcoR Ⅰ位点间1657-2125 nt的核苷酸序列为探针,同样与以上3份CMV中国分离物进行RNA杂交,进一步比较分析了这几个分离物与我国亚组Ⅰ的K-CMV 株系的关系,证明了我国CMV存在亚组与株系分化。更多还原

【Abstract】 cDNA sequences spanning 1209 nt to 1626 nt of Fny-CMV RNA2 (a subgroup Ⅰ strain) and 2002 nt to 2433 nt of Ls-CMV RNA2(a subgroup Ⅱstrain) were used for probe preparation. The 32P-labeled negative strand RNAs were prepared by transcription in vitro. Then 3 Chinese CMV isolates from pepper were purified and hybridized with RNA-probes respectively. The results showed that Beijing and Henan isolates share high sequence identities with Fny-CMV, belonging to CMV subgroup Ⅰ strain. Another isolate from Fujan province was belonged to CMV subgroup Ⅱ strain. Meanwhile, the purified RNAs of the 3 Chinese isolates were hybridized with a transcription probe complementary to the fragment flanking 1657nt to 2125nt of K CMV RNA2 (a subgroup Ⅰstrain originated from China). The differentiations of Chinese isolate were analyzed according to the results.更多还原