【作者】 王伟霞； 李福后；

【Author】 Wang Wei-Xia Li Fu-Hou (College of Marine Science,Huaihai Institute of Technology,Lianyungang 222005)

【机构】 淮海工学院海洋学院；

【摘要】 茯苓原生质体制备的最佳条件为:纤维素酶(1．5％)和蜗牛酶(1．5％)的等量混合酶解系统,酶解3h,7d菌龄菌丝,产量可达1.77×107个／ml。以甘露醇为稳渗剂,采用CYM再生培养基,酶解时间为2h,其原生质体再生率最高,为2．72 ×10-3。这一结果为茯苓通过原生质体技术进行菌种改良提供了重要技术参数。更多还原

【Abstract】 For the protoplast isolation of Wolfiporia cocos, the highest yield can be obtained by using 1.5% cellulose and snailase. The optimal age ofmycelia was 7d and optimal enzymolysis time was 3h. Th e highest protoplast yield was1.77× 107 / ml. When 0. 6mol / L mannitol was used for stabilizer of the regeneration medium Of CYM, the regeneration frequency of protoplast was2. 72 ×10-3. The results and data would provide important parameters for the strain improvement of Wolfiporia cocos through the protoplast technique.更多还原