Changes in expression profiles of sub-proteome in rat hippocampal CA1 region after forebrain ischemia

【作者】 陈亮； 杨建明； 李晓明； 高天明；

【机构】 南方医科大学；

【摘要】 <正>To explored the proteomic change in CA1 subregions of rat hippocampus between sham-operation rats and rats subjected to 15 min forebrain ischemia, we established an improved microdissection method and subproteomic profiling analysis for CA1, by using protein solubility-based sequential sample extraction and modified two-dimensional electrophoresis, which displays cytosolic or membrane proteins/peptides respectively. CA1 membrane proteins had been 2.5 fold enriched, which contribute to the more detailed integrated map containing more than 2500 spots. After analyzing using professional更多还原