【作者】 朱鲁生； 辛承友； 王倩； 刘爱菊； 王军； 谢慧； 王秀国；

【Author】 ZHU Lu-sheng, XIN Cheng-you, WANG Qian, LIU Ai-ju, WANG Jun, XIE Hui, WANG Xiu-guo (College of Resources and Environmental Sciences, Shandong Agricultural University, Taian 271018,China)

【机构】 山东农业大学资源与环境学院；

【摘要】 以莠去津降解细菌HB-5作为研究对象,探索了降解菌HB-5的固定化方法,并对降解菌HB-5及其固定化菌对土壤中莠去津的降解能力进行了比较。采用注射器滴定方法对细菌HB-5进行了固定化,并对海藻酸钠、聚乙烯醇、卡拉胶、脱乙酰壳多糖四种包埋材料从成本、固定时间等方面进行了比较,筛选出最佳固定条件。研究确定海藻酸钠为最佳包埋剂,并对包埋剂的浓度、包埋粒径等进行了探索。测定了降解菌HB-5及其固定化菌对土壤中莠去津的降解率。实验结果表明:以浓度为2.5%～3.5%的海藻酸钠溶液作为包埋剂进行固定化,生产的HB-5固定化菌机械强度和物理稳定性好,分离度高,符合实验和应用的要求。菌株经固定化后,降解莠去津的能力大大提高,在第1d以后,其降解率均高于未固定化菌;在第1~7d,随培养时间的延长,降解菌与固定化菌HB-5的降解率迅速提高,在第7d达到85%;在第7~13d,二者的降解率增长缓慢,但在第10d均可达到90%。更多还原

【Abstract】 The high-efficiency atrazine-biodegrading bacterium from industrial sewerage, named as HB-5, was filtrated. The method of immobilization of bacterium HB-5 were studied. The differences of biodegradation data between bacterium HB-5 and immobilized HB-5 on soil were compared. Bacterium HB-5 was cultured in basic liquid inorganic medium at 30℃, 48h with 5% inoculation. The bacterial pellet was sonificated and centrifuged at 8000r?min-1 for 6min at 15℃. After washed, wet thallis were put into refrigeratory at 4℃. Processed by iatrical syringe titration method, and compared four kinds of embedding material: sodium alginate, poly vinyl alcohol, carrageenan and chitosan in price and other factors. Finally, sodium alginate was confirmed as the best embedding material. Concentration of sodium alginate and diameter of immobilized HB-5 were also experimentalized. Besides, the capacity of bacteria HB-5 and immobilized HB-5 to degrade Atrazine on soil was determined. The results indicated that the particle manufactured by 2.5%～3.5%sodium alginate as embedding material with 4# iatrical syringe pinhead can satisfied the requirement of the dissertation, because of its preferably mechanism intensity and physics stability. It has been found that the capacity of bacteria HB-5 to degrade Atrazine was superior to the immobilized HB-5’s at the first day by gas chromatogram, but from the 3rd day to the 13th day, just the reverse. The degradation rates of both were increased dramatically with the days prolonged, they were got to 85% on the 7th day. But from the 7th day to the 13th day, the degradation rates were added to slowly. However, they were reached 90% on the 10th day.更多还原