节点文献
高效毛细管电泳法测定二氢叶酸还原酶活力的研究
Determionation of The activity of Dihydrofolate Reductase by High Performance Capillary Electrophoresis
【Author】 Rui Jia, Na Li , Ruo-hua Zhu (Department of Chemistry, Capital Normal University, Beijing 100037)
【机构】 首都师范大学化学系；
【Abstract】 MEKC has been applied for the study of the separation of dihydrofolate, tetrahydrofolate, NADP, NADPH and dihydrofolate reductase in the enzyme reaction system. The five components were separated in 18min by 50mmol/L disodium tetraborate (pH9.18) buffer solution with 0.002% Brij-35.The method was developed to determine dihydrofolate reductase activity by monitoring its product, tetrahydrofolate.
- 【会议录名称】 中国化学会第二十五届学术年会论文摘要集（下册）
- 【会议名称】中国化学会第二十五届学术年会
- 【会议时间】2006-07
- 【会议地点】中国吉林长春
- 【分类号】O658.9
- 【主办单位】中国化学会