【作者】 郭绍芬； 郭晓珊； 陆天虹； 周青； 黄晓华；

【Author】 Shao-Fen Guo1,2, Xiao-Shan Guo2, Tian-Hong Lu1,Qing Zhou2, Xiao-Hua Huang1,2* 1College of Chemistry and Environmental Science, Nanjing Normal University, 210097, Nanjing 2School of Biotechnology, Southern Yangtze University, 214036,Wuxi

【机构】 南京师范大学化学与环境科学学院； 江南大学工业生物技术教育部重点实验室；

【Abstract】 The interaction mechanism between RE(III) and HRP was investigated with the biophysical and chemical methods and cytochemical techniques, such as autoradiography, liquid scintillation counting, electron microscopy etc. The experimental results indicated that when RE(III) concentration is as low as10ppm, the interaction between RE(III) and HRP is weak, leading the change in the conformation of HRP, the exchange of Ca(II) in the cell and the slight change in the cell ultrastructure. It results in that the activity of HRP was increased by 21.2%. When the RE(III) concentration is as high as 80ppm, the interaction between RE(III) and HRP is strong, leading the significant change in the HRP conformation and the excessive exchange of Ca(II). Thus, the cell ultrastructure was significantly damaged the HRP activity is decreased by 30.8%. The different RE(III) show the different interaction ways and degree with HRP.更多还原