【作者】 王崇龙； 薛仁宇； 曹广力； 沈卫德； 贡成良；

【Author】 Wang Chonglong, Xue Renyu, Cao Guangli, Shen weide, Gong Chengliang Life Science School, Soochow University, Suzhou, 215123

【机构】 苏州大学生命科学学院；

【摘要】 本文克隆了家蚕核型多角体病毒苏州株碱性核酸酶(AN)基因及其侧翼区,并进行序列和进化分析。 BLAST检索显示,BmNPV AN上游的ORF109部分序列仅在AcNPV、RoNPV有同源序列:下游区域在AcNPV、 RoNPV、SINPV的基因组中检索到同源序列,并存在2个正向的重复;BmNPV-SU的AN基因编码420个氨基酸残基,比对结果分析显示,在22种杆状病毒中,AN基因间核苷酸序列和氨基酸序列的同源性较低,变异较大。同时,利用MEGA软件包中的UMEGA算法建立了基于AN基因的分子进化系统树,表明杆状病毒与其宿主昆虫之间存在共进化关系。更多还原

【Abstract】 alkaline nuclease(AN) and its flanking region of Bombyx mori Nucleopolyhedrovirus Suzhou strain was cloned, sequenced, and analyzed in this study. According to the BLAST research result, the homologues of part of ORF109 on the upstream of BmNPV AN gene had been found only in AcNPV and RoNPV, and the downstream region, which contented two direct repeated sequence, had in AcNPV, RoNPV, and S1NPV. BmNPV AN gene encoded 420 amino acids. Alignment between 22baculoviruses showed that neither nucleotide nor amino acid sequenci of AN gene homogeneity was low, variation was high. Phylogenetic tree of AN gene, which was constructed using UMEGA method in MEGA software, revealed that baculovirus and their hosts were coevolved.更多还原