【作者】 唐斌； 徐世清； 戴璇颖； 陈息林； 司马杨虎； 王玉军； 吴阳春；

【Author】 TANG Bin, XU Shiqing, DAI Xuanying, CHEN Xilin, SIMA Yanghu, WANG Yujun Wu Yangchun School of Agricultural Science and Technology & Institute of Sericulture, Soochow University,Suzhou 215006

【机构】 苏州大学生命科学学院苏州大学蚕桑研究所；

【摘要】 养蚕生产使用一代杂交种,家蚕品种纯度依靠幼虫斑纹或茧形等形态鉴别,该方法易受环境影响,时效性和准确性差。利用单粒卵微量DNA抽提法,从江苏省主要家蚕品种苏5、苏6及其F1孵化前日卵快速提取出基因组DNA。用筛选出的RAPD随机引物S42稳定扩增出2个亲本品种的DNA特异性片段R5和R6,克隆和测序后大小分别为255bp和343bp。网上比对结果,R5的nt7-192与一个家蚕非长末端逆转录转座子基因的 nt183-368片段碱基序列有高达91％的相似性。R6的nt5-340与一个家蚕WGS的nt675-337有91％的一致性, 其中存在13个Gaps。将2个亲本的RAPD标记转换成SCAR标记,分别利用合成的S42-255-2(P5-2)和S42-343-2 (P6-2)SCAR标记引物,能够准确、快速地鉴别所标记的苏5和苏6及其F1蚕品种。更多还原

【Abstract】 Sericulture production is used to the first filial generation. The rule method to identify silkworm variety and purity with morphological characters of larval pattern or cocoon shape is easily affected by environment. Thus, the prescription and accuracy is low. The genome DNA of silkworm was extracted from single blue-stage-egg of mainly silkworm varieties in Jiangsu province of SU5, SU6 and their Flwith rapid method. The specific bands R5 and R6 of RAPD were amplify traced each from of SU5 and SU6 variety, with selected random primer of S42. The sequences were 255bp and 343bp respectively after cloned and sequenced. Then analyze the sequences by courtesy of database on-line. The results showed the nt7-192 of R5 and the nt183-368 of the non-LTR retrotransposon of silkworm has 91% consistency. Accordingly, 91% of nt675-337 of one of whole genome shotgun sequence (WGS) of silkworm shows the same sequence as the nt5-340 of R5, which include 13 gaps. Further, transform the RAPD mark of two parental varieties to SCAR mark. Thus, SU5, SU6 and their F1 were identified accurately and fast, with the synthetic specific primers of S42-255-2 (P5-2) and S42-343-2 (P6-2) .更多还原