【作者】 叶妙水； 钟克亚； 张桂和； 胡新文； 郭建春；

【Author】 Ye Miaoshui Zhong Keya Zhang Guihe Hu Xinwen Guo Jianchun State Key Laboratory of Tropical Crops Biotechnology, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical A- gricultural Sciences, Haikou, 571101

【机构】 中国热带农业科学院热带生物技术研究所热带作物生物技术国家重点实验室；

【摘要】 植物Na+／H+逆向运输蛋白在植物耐盐性方面起着极为重要的作用。根据同源序列设计引物, 通过RT-PCR方法从真盐生植物北美海蓬子(Salicornia bigelovii)中克隆出包括1 683bp的完整编码区在内的2 591bp(GenBank登陆号为DQ157454)的Na+／H+逆向运输蛋白基因(NP)cDNA序列。将该基因编码区序列构建到植物表达载体pVKH-35S-pA上,并通过农杆菌介导转化到模式植物拟南芥(Ara- bidopsis thaliana)中,得到T3代转入单基因种子。200mmol／L的NaCl胁迫培养结果显示,转基因植株生长状况好于野生型植株。更多还原

【Abstract】 Na+/H+ antiporter plays an important role in plant salt-tolerance. We designed primers according to the homologous gene region. The cDNA (DQ157454) is 2 591bp in length including an open reading frame of 1 683bp which we had achieved by RT-PCR from Sdicornia bigelovii. We had inserted the translated region of gene to plant expression vectors of pVKH-35S-pA, and then transgene to Arabidopsis thaliana by Agrobacterium-mediaied. The transgenic plants were grown better than wild-type plants in the presence of 200 mmol/L sodium chloride.更多还原