【作者】 杨佑明； Yingru Wu； Danny J Llewellyn； 李召虎； 徐楚年； Elizabeth S Dennis；

【Author】 YANG You-ming , Yingru Wu, Danny J Llewellyn ,LI Zhao-hu ,XU Chu-nian, Elizabeth S Dennis ( Cotton Research Centre, College of Agronomy & Biotechnology, China Agricultural University, Beijing 100094, China; CSIRO Plant Industry, GPO Box 1600, Canberra, ACT 2601, Australia)

【机构】 中国农业大学棉花研究中心中国农业大学农学与生物技术学院； CSIRO Plant Industry,GPO Box 1600,Canberra,ACT 2601,Australia；

【摘要】 利用BAC克隆直接测序法克隆了GhMyb25的基因组序列,其中,cDNA5’非编码区上游序列中有2个TATA盒、1个CCAAT盒和5个HD蛋白结合位点等。将长1756 bp的GhMyb25开放读码框上游序列与GUS基因融合,用粒子轰击法转化棉胚珠,结果GUS基因在棉纤维细胞内得到表达, 说明该序列具有GhMyb25启动子功能。还讨论了粒子轰击法转化、结合胚珠离体培养方法的应用前景。更多还原

【Abstract】 The genomic sequence of GhMyb25 was revealed by direct sequencing of BAC clones. The 1690bp upstream sequence of its 5 ’ lead sequence contains 2 TATA boxes, 1 CCAAT box and 5 homeodomain protein sites, etc. A new plasmid, GhMyb25P; ;GUS was constructed by fusing the upstream sequence of GhMyb25 open reading frame with GUS gene. Cotton ovules on the day of anthesis were bombarded with gold particles attached GhMyb25P; ;GUS. The transformed ovules were cultured and the GUS gene was expressed in fiber cells. It was clear that the 1690bp upstream sequence of GhMyb25 5’ lead sequence shown its promoter function. It is discussed the applied prospect of cotton ovules transformed by particle bombardment.更多还原