【作者】 张飚； 张凤蕴； 赵育莹； 王丽群； 周贵生； 于伟玲； 李殿俊；

【Author】 ZHANG Biao,ZHANG Feng-yun,ZHAO Yu-ying,et al (Department of Immunology, Harbin Medical University, Harbin 150086, China)

【机构】 哈尔滨医科大学免疫学教研室；

【摘要】 目的探讨胸腺内注射异基因抗原在延长移植皮片存活期中的作用。方法经破碎、超速离心、透析等步骤自C57BL/6小鼠脾细胞提取MHC(H-2)抗原,注射到Balb/c受体小鼠遥腺内,1周后移植供体C57BU6皮肤。共设实验组、第三供体组、对照组、无处理组4组。结果实验组皮肤移植物存活时间大于70天,第三供体组为(13．4 ±1．42)天,对照组为(12．6±1．69)天．无处理对照组为(5．6±1．26)天。实验组与对照组相比有显著性意义(P<0． 01)。结论经胸腺注射异基因MHC抗原能诱导特异性皮肤移植耐受。更多还原

【Abstract】 Objective to study the effects of intrathymic injection of allogenic antigen on prolongation survival of skin grafts. Methods MHC antigen was extracted from C57BL/6 mouse splenocytes through destruction, ultrafugation, dialysis and was intrathymically injected into Balb/c recipients mouse, a week after the injection, C57BL/6 skin transplatation was performed. The experiment was divided into four group, the experimental group, the third-part group, the control group and the unconditional control group. Results The median survival time(MST) of skin allografts in the experimental group was over 70 days,in the third-part group( 13.4 ± 1. 42) days,in control group(12.6± 1.69) days,in the unconditional control group(5.6± 1.26) days.The median survival time was significantly prolonged in the experimental group (P < 0.01). Conclusion The intrathymic injection of donor MHC antigen extracted from C57BL/6 could induce recipients specific tolerance to skin transplantation.更多还原