野生小花溲疏繁殖方法研究

【作者】 史宝胜； 刘冬云； 杨新兵； 杜鸿云； 张彦广；

【机构】 河北农业大学园林与旅游学院；

【摘要】 研究了野生小花溲疏种子发芽、硬枝扦插和组织培养条件,结果表明:种源地对溲疏种子萌发有较大影响,以涉县的种子发芽率最高,达83．1%;溲疏种子最适发芽温度为 20℃。在不同年龄段的枝条扦插生根试验中,以二年生的溲疏枝条扦插生根率最高,达 40．65％;用1 000mg·L-1IBA溶液速蘸插条基部,其生根率最高为69．85％;溲疏组织培养适宜培养基类型分别为:①初代培养基:MS+6-BA 2．0 mg·L-1+NAA 0．1 mg·L-1;②继代培养基:MS+6-BA 2．0 mg·L-1+NAA 0．1 mg·L-1;③生根培养基:1／2 MS+IAA 1．0 mg·L-1。这些条件的确定为今后大量生产溲疏种苗奠定了基础。更多还原

【Abstract】 We studied the germination rate of the Deutzia scabra Thunb, the colne propagation of cutting and tissue culture. The results showed that the rate of germination were diversity among places. The highest rate was shexian with reaching 83.1%. The optimum temperature was around 20 ℃ for the seed germination of wild Deutzia Scabra Thunb. Two years shoots could take more roots than other aging shoots which reached 40. 65%. Using 1 500mg · L-1 IBA fast dipping could take rootage which reached 69. 85%. The fitting culture medium as fellow: The starting culture medium was MS+6 - BA 2. 0 mg ·L-1 +NAA 0.1 mg ·L-1; the optium medium for shoot - tip to differentiate was MS+6 - BA 2. 0 mg ·L-1 +NAA 0. 1 mg · L-1; The optimum medium for root induction was 1/2 MS+IAA 1. 0 mg ·L-1. These studies made the foundation for mass-producing Deutzia scabra Thunb seedling in the future.更多还原