【作者】 陈念； 高向阳； 林壁润；

【Author】 CHEN Mian,GAO Xiang-yang, LIN Bi-run(College of Life Sciences,South China Agricultural University ,Guangzhou,Guangdong 510642,China; Plant Protection Research Institute,Guangdong Academy of Agriculatural Science,Guangzhou,Guangdong 510640,China)

【机构】 华南农业大学生命科学学院； 广东省农科院植物保护研究所；

【摘要】 以革兰氏阳性细菌枯草芽孢杆菌MIGI.22(Bacillus subtilis)和革兰氏阴性细菌水稻白叶枯病菌(Xanthomonas ovyzaepy)为研究对象,对万隆霉素的抑菌机理进行了初步研究。结果表明.万隆霉索可显著抑制这2种细菌的氧呼吸作用,随着反应体系中万隆霉素终浓度由0逐渐增加到50 μg/mL,枯草芽孢杆菌氧呼吸作用持续的时间由对照的28 min减小到10 min,白叶枯病菌则由18 min减小到8 min,两者的相对耗氧量分别由对照的86.9%和62.9%逐渐下降到0;此外,对这2种细菌NADH氧化酶活力进行的研究表明,万隆霉素可显著抑制细菌各个生长阶段酶的活力的提高,这与万隆霉素抑制其氧呼吸作用密切相关。更多还原

【Abstract】 This paper investigated the mechanism of Wanlongmycin against two typical sensitive bacteria-Bacillus subtilis (G+) and Xanthomonas ovyzaepy (G-).The oxygen electrode experiment indicated that the inhibition effect of Wanlongmycin on the O2 respiration of bacteria was obvious. With the concentration of Wanlongmycin increasing from 0 to 50 μg/mL,the duration of the O2 respiration of Bacillus subtilis and Xanthomonas ovyzaepy decreased from 28 min to 10 min and 18 min to 8 min, respectively. Their relative oxygen consumption quantity gradually decreased from 86. 9% and 62. 9% to 0. The change of NADH oxidase activity of bacterial showed that Wanlongmycin could inhibit the enzyme directly during the entire bacteria growth period. This O2 respiration inhibition mechanism may play an important role in the inhibition effect of Wanlongmycin against bacteria.更多还原