【作者】 聂开立； 谭天伟； 王芳；

【Author】 NIE Kaili TAN Tianwei WANG Fang(Department of Biochemical Engineering, College of Life and Science Technology,Beijing University of Chemical Technology, Beijing 100029, People’s Republic of China)

【机构】 北京化工大学生命科学与技术学院北京市生物加工过程重点实验室；

【摘要】 探讨了利用本实验室自制的Candida sp99-125脂肪酶转酯化合成生物柴油的过程。利用固定床反应器生产生物柴油,经过初步优化的试验结果表明,在采用分级流加甲醇和旋液分离技术的工艺条件下,采用色拉油为地物生产生物柴油的转化率可以达到93%左右,采用废油为地物,转化率可达到92%以上。固定化酶的使用寿命大约500小时(20天)。所生产的生物柴油粗产品经过刮板膜蒸发器分离精制后产品收率高于85%。所得到的生物柴油产品的各项指标均符合德国生物柴油生产标准(DIN 51606-97)。更多还原

【Abstract】 Lipase catalyzed soybean oil to produce biodiesel fuel in fixed-bed bioreactor was studied in this paper. Three-step transesterification with methanol (methanolysis) of oil was conducted using columns packed with immobilized Candida sp.99-125 lipase. Hydrocyclones were used to separate the by-product glycerol on-line after every 1/3 molar equivalent of methanol. The whole operation can therefore be operated continuously by this method. The final conversion of the FAME from plant oil and waste oil under the optimal condition was 93% and 92% respectively. The life of the immobilized lipase was more than 20 days. After distillation the product could reached the standard of biodiesel in Germany (DIN 51606 97).更多还原