【作者】 郭润芳； 李多川；

【Author】 Guo Rtmfang~(1,2) Li Duochuan~1 1 Department of Environmental Biology,Shandong Agricultural University,Tai’an,271018;2 Department of Bioengineering,Agricultural University of Hebei,Baoding,071000

【机构】 山东农业大学环境生物系；

【摘要】 根据Thermomyces lanuginosus热稳定几丁质酶Chi48的N-端氨基酸序列和同源保守序列设计简并引物,通过RT-PCR 及快速扩增cDNA 末端(RACE)的方法,克隆了该几丁质酶的编码基因chit,全长cDNA 为1 500bp,包含一个由442个氨基酸组成的开放阅读框。该基因已在GenBank 中注册,登录号为DQ092332。比较推导的氨基酸序列,发现其催化区与18家族几丁质酶的同源性很高,包含两个与催化活性有关的保守基序。更多还原

【Abstract】 A chitinase-encoding gene from Thermomyces lanuginosus was cloned by Rapid Amplification of cD-NA Ends(RACE)using degenerated oligonucleotide primers designed from N-terminal amino acid sequence andthe conserved amino acid sequence.The cloned full-length cDNA named chit,1 500bp in size,contained an OFRwith 442 amino acids.The gene chit has been registered in GenBank with accession number DQ092332.Thealignment results of putative amino acids sequence showed the catalytic domain of chit was high homology withthe catalytic domains of the other chitinases in family-18,contained 2 conserved motifs related with catalytic ac-tivity of chitinase.更多还原