【作者】 陈明； 孙昌魁； 程剑锋； 李强； 张玉臻；

【Author】 Chen Ming, Sun Changkui, Cheng Jianfeng, Wang Ximeng , Zhang Yuzhen (State Kkey Llaboratory of Mmicro- biotechnology, Shandong University, Jinan 250100)

【机构】 山东大学微生物技术国家重点实验室；

【摘要】 几丁质酶高产菌株黏质沙雷氏菌L15-2在含有1%胶体几丁质、0.3%K2HPO4、0.3%KH2PO4、0.05%MgSO4、0.05%CaCl2、0.001%FeSO4的产酶培养基中37℃培养4 天后,粗酶液经过DEAE Sepharose Fast Flow和Sephadex G-100,电泳检测得到电泳纯的几丁质酶。该酶的性质特征测定结果表明,该几丁质酶的分子量为41.314kD;最适作用温度为50℃,最适作用pH为6.6:在60℃之前热稳定性较好,在pH4-10范围内较稳定;各种金属离子对酶活力影响不同,Fe2+、Zn2+抑制作用明显,而Ba2+、Mn2+、Ca2+对酶活性有一定的促进作用。几丁质酶对致病真菌的抑制作用也很明显。更多还原

【Abstract】 Serratia marcesens L15-2 which produces chitinase highly was isolated by the double layer plates containing chitin. The optimal fermentation medium composed 1% chitin, 0.3% K2HPO4, 0.3%KH2PO4, 0.05%MgSO4, 0.05%CaCl2, 0.001%FeSO4. After fermentation for 4 days at 37℃ and purification, the purified chitinase can be tested by SDS-PAGE. The initial temperature is 50℃ and pH is 6.6, The enzyme is stable below 60℃ and between pH 4-10. Different metal ions show different effects on the enzyme activity. The chitinase can be enhanced by Ba2+, Mn2+ and Ca2+ and inhibited by Fe2+ and Zn2+. The chitinase also has an obvious effect on restraining the growth of epiphyte.更多还原