【作者】 秦丽娟； 曹宇；

【Author】 QIN Li-juan~2, CAO Yu~(1) (Department of physiology, China Medical University, Shenyang 110001, China)

【机构】 内蒙古医学院生理教研室； 中国医科大学生理学教研室；

【摘要】 目的:探讨热应激预处理诱导产生的热休克蛋白70对肝脏缺血/再灌注损伤的保护作用的机制。方法:应用pringle,s法制备肝脏缺血/再灌注损伤模型及热应激预处理模型。将实验大鼠随机分为热应激预处理(HP+I/R)组与非预处理(I/R)组,对比观察两组动物肝脏缺血/再灌注后0、4、8、12、24h时肝脏HSP70的表达、SOD活力和MDA的产生量及大鼠血清门冬氨酸转氨酶(aspartatetransaminase,AST),丙氨酸转氨酶(alaninetransaminase,ALT)的活性与肝脏病理组织学改变。结果:热应激预处理组各时间点肝脏HSP70的表达及SOD的活力均比非预处理组同一时间点高,而血清AST、ALT酶活性及MDA的产生量较非预处理组低,病理损伤也比非预处理组减轻。结论:热应激预处理诱导产生的热休克蛋白70可能通过促进SOD的产生,从而降低氧自由基对肝脏的损害,起到保护肝脏缺血/再灌注损伤的作用。更多还原

【Abstract】 Aim: To study the protective mechanism of HSP70 induced by the heat stress pretreatment on the hepatic ischemia/ reperfusion(I/R) injury. Methods: To establish the models of the hepatic ischemia/ reperfusion injury using pringle’s maneuver with or without heat stress pretreatment. The rats were randomly divided into pretreatment group(HP+I/R) and non-pretreatment group(I/R), in which the expression of HSP70, the MDA contents and SOD activity in liver, the activities of serum AST and ALT, and the pathological changes in liver were detected at 0,4,8,12 and 24 h after I/R. Results: At any time point set after I/R, the SOD activity in the liver in HP+I/R group were higher than those in IR group, HSP70 expression maximum was attained at 12 h after heat pretreatment. While in HP+I/R group the levels of liver enzymes and the production of MDA significantly reduced and the pathological changes improved compared with those in I/R group. Conclusion: HSP70 induced by heat pretreated protecting liver against I/R injury may be through increasing the SOD content and then reducing the insults of oxygen free radicals in the liver.更多还原