【作者】 许传莲； 郑毅男； 韩立坤； 奥田拓道；

【Author】 XU Chuan-lian~1 ZHENG Yi-nan~2 HAN Li-kun~3 Okuda T~3 (1、Zhejiang University Of Science,Hangzhou,310018,China;2、College of Traditional Chinese Medicinal Material,Jilin Agriculture University,ChangChun,130118,China;3、Department of Bioenvirement K umamotohigashi University,Kumamotohigashi,862-8502,Japan)

【机构】 日本熊本县立大学环境共生学部； 浙江理工大学生命科学学院； 吉林农业大学中药材学院；

【摘要】 目的:研究大黄柳叶醇提取物及其黄酮类成分是否具有抑制由于喂饲高脂食料而引起的小鼠肥胖的作用。方法:用添加大黄柳叶醇提取物的高脂肪食料喂饲小鼠10周,测量小鼠体重、子宫周围脂肪组织重量以及肝脏中甘油三酯的含量;检测从大黄柳叶中分离得到新的黄酮化合物地奥亭7-O-β-D-吡喃木糖(1? 6)β-D吡喃葡萄糖苷在脂肪细胞中对去甲肾上腺素诱导脂肪分解的作用。结果:大黄柳叶醇提取物可以抑制喂饲高脂食料引起的肥胖和脂肪肝。其主要活性成分为黄酮化合物地奥亭7-O-β-D-比喃木糖(1? 6)β-D-吡喃葡萄糖苷,能够促进去甲肾上腺素诱导的脂肪分解,大黄柳叶醇提取物及单体化合物均无抑制胰脂肪酶活性的作用。结论:大黄柳叶的减肥作用可能是由于大黄柳叶中含有促进脂肪分解作用的黄酮化合物地奥亭7-O-β-D-吡喃木糖(1? 6)β-D吡喃葡萄糖苷及其它黄酮成分所致。更多还原

【Abstract】 Objective:The present experiment was constructed to clarify whether thealcohol extrae and flavoniod constituents from leaves of Salix raddeana Laksch prevented obesityinduced in mice by the oral administration of a high-fat diet.Method:High fat diet-induced obesemice were treated with the alcohol extract and flavoniod constituents from leaves of Salixraddeana Laksch for 10 weeks.We measured the weight of the body,uterine adipose tissue of miceand the content of triolein of the liver.The effect of the new flavonol glycoside doismetin 7-o-β-D-xylopyranosyl(1→6)-β-D-glucopyranoside on noradrenaline-induced lipolysis wereexamined with isolated fat cells.Results:The extract of Salix raddeana Laksch prevented obesityand fatty liver induced in mice by the oral administration of a high-fat diet.The active substancein the alcohol extract of Salix raddeana Laksch was identified as a new flavonal glycoside,whichenhanced norepinephrine-induced lipolysis in fat cells.The alcohol extract and flavonoidconsituents didn’t inhibit the activity of pancreatic lipase.Conclusion:It is demonstrated that theanti-obesity effects of the leaves of Salix raddeana Laksch on high-fat diet-treated mice may bedue partly to the enhancing effects of doismetin 7-0-β-D-xylopyranosyl(1→6)-β-D-glueopyranoside on norepinephrine-induced lipolysis in adipose tissue.更多还原