【作者】 沈洁； 丁小余； 张卫明； 唐凤； 刘冬扬； 贺佳；

【Author】 SHEN Jie DING Xiao-yu ZHANG Wei-ming LIU Dongyang HE Jia TANG Feng(College of Life Sciences, Nanjing Normal University, Nanjing 210097, China; Nanjing Institute for the Comprehensive Utilization of Wild Plants, Nanjing 210042, China)

【机构】 南京师范大学生命科学学院； 南京野生植物综合利用研究院；

【摘要】 目的:研究不同居群的花椒及其混淆品的rDNA ITS区碱基序列的特征及其差异,为花椒的鉴别提供可靠的分子标记。方法:运用PCR产物直接测序和克隆测序法对来自于甘肃、陕西、四川、河北等地的7个花椒居群及3个混淆种的rDNA ITS区(包括ITS1,5.8S,ITS2)碱基序列进行了测定。结果:首次报道了花椒ITS区的碱基序列,序列总长度为619～620bp,长度变异较少,与混淆种长度仅相差4bp。花椒各居群中,rDNA ITS区碱基序列有15个变异位点、12个信息位点、3个特异性识别位点。与混淆品间的碱基差异则较为显著,多达71个变异位点,有4个花椒特异性识别位点。结论:依据花椒ITS区的序列特征可以准确鉴别各居群的花椒及其混淆品;亲缘关系密切的花椒居群在地理位置上也非常靠近;rDNA ITS序列特征可作为花椒种内和种间鉴别的有效分子标记。更多还原

【Abstract】 AIM: To study the difference of rDNA ITS sequences among Zanthexylum bungeanum populations and their adulterants in main habitants of China so as to provide molecular markers for identifying Z. bungeanum populations from adulterants. METHODS: rDNA ITS regions (including ITS-1, 5. 8S and ITS-2) of 7 populations of Z. bungeanum which are respectively located in Gansu, Shaanxi, Sichuan, Hebei provinces, and 3 adulterants were sequenced by PCR products sequencing method or clone sequencing method. RESULTS: The sequences of rDNA ITS region of Z. bungeanum were reported for the first time, and the sequences of ITS region ranged from 619 to 620bp, and the length difference among Z. bungeanum and their adulterants is 4bp. There are 15 variable sites, 12 informative sites and 3 authenticable sites among Z. bungeanum populations. The difference of rDNA ITS regions among Z. bungeanum and their adulterants are obvious, the number of variable sites is 71. CONCLUSION: The difference of rDNA ITS sequences can be used to authenticate accurately the populations of Z. bungeanum and their adulterants. Those populations of Z. bungeanum which have close relationship always distribute in near geographic areas. The characteristics of rDNA ITS sequence can be used as good markers for authenticating Z. bungeanum populations from their adulterants.更多还原