【作者】 杨红梅； 徐琴； 张文平； 吾甫尔·米吉提； 艾尔肯·热合曼；

【Author】 YANG Hong-mei;XU Qin;ZHANG Wen-ping;GH·MIJIT;E·RAHMAN College of Life Science and Technology,Xinjiang University,Urumqi, Xinjiang 830046, China

【机构】 新疆大学生命科学与技术学院；

【摘要】 用微玻璃珠研磨法将p387NIT1质粒转入到莱茵衣藻硝酸还原酶缺陷型及缺壁突变株品系CC-2677(cw15nit1-305mt-)中,得到了一些不同的插入型实变转化子,在本实验室条件下CC-2677突变株品系的转化率最高时达到了88个/μgDNA,经多次转化共获得各种转化子1800多个,对它们进行荧光检测后检测出缺乏叶绿素b的一个突变子NIT17-1,并进一步用高效液相色谱法对其光合色素含量进行检测,结果证实该突变体的确丧失了叶绿素b合成能力,该突变株将可作为CBN1基因克隆及其定位的有利工具。更多还原

【Abstract】 The plasmid p387NIT1 was transformed into nitratase deficient and cell wall-less strain CC-2677(cw15nit1-305mt-) of Chlamydomonas reinhardtii by using glass bead method, and some insertion mutatedtransformants with various background have been obtained. The highest transformation rate of the CC-2677 is88 per μg DNA. At last abovel 800 transformants have been obtained and fluorescence test was performed toscreen them, from which one chlorphyll b-less mutant NIT17-1 has been detected and isolated. The further re-sult of detection of photochrome contents screened by HPLC method demonstrated that the mutant had lost theability of biosynthesis chlorophyll b. and therefore it will becoming useful tool for the cloning and localization ofthe CBN1 gene.更多还原