【作者】 李延； 尹大强； 赵庆顺；

【Author】 Li Yan Yin Daqiang Zhao Qingshun School of the Environment, State Key Laboratory of Pollution Control and Resource Reuse,Nanjing University, Nanjing, 210093 Model Animal Research Institute of Nanjing University, Nanjing, 210089

【机构】 南京大学环境学院污染控制与资源化研究国家重点实验室； 南京大学模式动物研究所；

【摘要】 本研究将斑马鱼经五氯酚(PCP)体内暴露染毒10天,提取肝脏基因组DNA。通过巢式PCR-限制性片段长度多态性方法(nested PCR-RFLP)检测包含斑马鱼p53基因第2,3,4外显子及其间的两个内含子的片段上的点突变。使用六种限制性酶,共检测样本20例,但均未检测到点突变。结果表明此六个酶切位点不是p53基因点突变的热点;nested PCR-RFLP方法具有快速简便等特点,可用于点突变的检测。更多还原

【Abstract】 The hepatic genomic DNA of zebrafish was extracted after exposure to pentachloroph-enol 10d in vivo. Point mutations on a fragment contained exon2, 3, 4 and two whole introns among them of p53 gene were detected by nested PCR-RFLP assay. Six restriction enzymes were used and 20 samples were detected, but no point mutation was found at the cut site. Results show that these six restriction enzyme cut sites are not the hotspots of p53 gene point mutation; nested PCR-RFLP assay can be used to detect point mutation because it is fast and convenient.更多还原