【作者】 计亮年；

【Author】 Ji Liang-nianThe School of chemistry and Chemical Engineering, The Key Laboratory of Gene Engineering of Ministry of Education, State Key Laboratory of Optoelectronic Material and Technologies, Zhongshan University, Guangzhou 510275, China, c

【机构】 中山大学化学与化学工程学院教育部基因工程重点实验室光电材料与技术国家重点实验室；

【摘要】 <正> 本报告分为以下五部分:第一部分是引言,它致力于介绍研究本课题的目的和意义,我们将在此汇报我们实验室的最近工作。第二部分是设计、合成一系列新的配体,以及它们的各种金属配合物,我们兴趣在设计和合成不同大小、几何形状,以及含有各种推电子或吸电子基团的多吡啶、大环和卟啉配体以及它们的各种金属配合物,为了使插入配体能插入DNA,更多还原

【Abstract】 This report is divided into following five sections, the first section is introduction, it is devoted to introduce the purpose and significance of the studies of the project. We shall report in here the recent work from our own laboratory. The second section, a series of new ligands and their many different metal coordination compounds were designed and synthesized , We have been interested in the design and synthesis of different size, geometry and with various electro-donor or acceptor groups of polypyridine, macrocyclic, and porphyrin ligand and their many different metal coordination compounds. In order to make the intercalated ligand can intercalate in DNA, the intercalative ligand generally needs flat, almost rigid large surface area, fused six membered terocycles and a special geometry that permits stacking or overlapping between aromatic ring of the intercalative ligand and the base pairs in DNA. These complexes were characterized by elemental analysis, UV-visible absorption spectroscopy, emission, IR, mass spectra, 1H and 13C NMR, cyclic voltammetry, X-ray and so on. All experimental values of coordination compounds are in nice agreement with the theoretical values. The third section is the review the history of the flurry of the new research field. We introduce in here the present heated debate focuses on binding mechanism of coordination compounds for DNA. Namely, whether [Ru(phen)3]2+ coordination compound intercalated or non-intercalated into between the base pairs of DNA? The fourth section is the discussion of the studies on binding mechanism and influence factors of interactions of coordination compounds with DNA by kinetics, thermodynamics and DFT calculation methods, including (1) kinetics and binding mechanism of A and A chiral coordination compounds with DNA; (2) The direct proportion of regulars of between the intercalated ligand planarity and their area size with DNA binding strength; (3) electronic effects of substitution group on the intercalated ligand; (4) effects of the ancillary ligands; (5) influence of the intercalated ligand space configuration and hydrophobicity; (6) effect of intramolecular hydrogen binding of the intercalated ligand; (7) effect of the intercalated ligand shape for selective binding DNA; (8) the steric hindrance influence of the intercalated ligand; (9) stereoselectivity of coordination compound [Ru(phen)3]2+ binding in different native DNA; (10) effect of the substitution position in the intercalated ligand; (11) enantiopreferential DNA-binding of dinuclear and polynuclear coordination compounds and so on. The final section go a step further to report biological function of complexes and their application exploration. These coordination compounds can be regarded as including (1) the structure probes for DNA; (2) A series of novel molecular "light switch" for DNA; (3) photocleavage agents of DNA; (4) the antitumor activity of novel ruthenium coordination compounds; (5) luminescence pH sensor and so on. The regulars among the structure of coordination compounds, the interaction mechanism and biological function was suggested further by us. The binding behaviors and biological functions of coordination compounds for DNA can be modulated and changed by the change of structure of coordination compounds.更多还原