【作者】 竞花兰； 姜富学； 成建定； 张有宾； 张宜骏； 李章旺； 吴金浪；

【Author】 JING Hua-lan, JIANG Fu-xue, CHENG Jian-ding, et al. ~△ Department of Forensic Pathology, Preclinical school of Sun, Yat-sen University, Guangzhou 510080, China)

【机构】 中山大学基础医学院法医学系； 中山大学基础医学院电镜室；

【摘要】 目的观察电击致死大鼠的心、脑、肺、皮肤超微结构形态学改变,寻找电击死的法医学鉴定依据。方法大鼠12只,其中电击致死6只,另6只对照;取心、脑、肺、皮肤,用戊二醛固定、锇酸染色后透射电镜观察。结果所取组织细胞均见明显细胞凋亡样改变,其中红细胞肿胀变形,充填整个毛细血管管腔。结论心、脑、肺、皮肤组织细胞与血管内皮细胞的超微结构形态改变对鉴定电流死有参考价值。更多还原

【Abstract】 Objective To detect the morphological evidence of identifying death from electrical injury, the ultrastructural changes of the heart, brain,lung and skin in the electrocuted rats were observed by transmission electron microscopy (TEM). Methods 12 rats were divided into the experimental and control groups. In the experimental group,6 rats were killed by electrocution, and in the control group, 6 rats were killed by decapitation. Samples from the heart, brain, lungs, and skin were fixed with valeric aldehyde and post-fixed with osmic acid. After being stained, the ultrathin sections were observed by TEM. Results Evident cell apoptosis was detected in all the tissues examined, and erythrocytes appeared swelling, which stuffed the lumen of the capillary vessels. Conclusion The ultrastructural changes of the cells of the heart, brains, lungs, and skins including the vascular endothelial cells may be used as the indicators for identifying death from electrical injury.更多还原